Microscopic Insight into the Protein Denaturation Action of Urea and Its Methyl Derivatives

The Journal of Physical Chemistry Letters
Bei DingFeng Gai

Abstract

We employ site-specific, linear and nonlinear infrared spectroscopic techniques as well as fluorescence spectroscopy and molecular dynamics simulations to investigate the binding interactions of urea and three of its derivatives, methylurea, 1,3-dimethylurea, and tetramethylurea, with protein aromatic and polar side chains. We find that (1) urea methylation leads to preferential interactions between the cosolvent molecules and aromatic side chains with an affinity that increases with the number of methyl groups; (2) interactions with tetramethylurea cause significant dehydration of aromatic side chains and the effect is most pronounced for tryptophan; and (3) while neither urea nor tetramethylurea shows preferential accumulation around a polar side chain, the number of hydrogen-bond donors around this side chain is significantly decreased in the presence of tetramethylurea. Taken together, our findings suggest that these urea derivatives, especially tetramethylurea, can effectively disrupt hydrophobic interactions in proteins. Additionally, tetramethylurea can promote intramolecular hydrogen-bond formation and hence induce α-helix folding in peptides, as observed.

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Citations

Sep 12, 2019·Physical Chemistry Chemical Physics : PCCP·Bogdan A Marekha, Johannes Hunger
May 20, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Ravi Kumar Venkatraman, Carlos R Baiz

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