Microscopy-Based Automated Live Cell Screening for Small Molecules That Affect Ciliation

Frontiers in Genetics
Peishan ZhangErica A Golemis

Abstract

The primary monocilium, or cilium, is a single antenna-like organelle that protrudes from the surface of most mammalian cell types, and serves as a signaling hub. Mutations of cilia-associated genes result in severe genetic disorders termed ciliopathies. Among these, the most common is autosomal dominant polycystic kidney disease (ADPKD); less common genetic diseases include Bardet-Biedl syndrome, Joubert syndrome, nephronophthisis, and others. Important signaling cascades with receptor systems localized exclusively or in part at cilia include Sonic Hedgehog (SHH), platelet derived growth factor alpha (PDGFRα), WNTs, polycystins, and others. Changes in ciliation during development or in pathological conditions such as cancer impacts signaling by these proteins. Notably, ciliation status of cells is coupled closely to the cell cycle, with cilia protruding in quiescent (G0) or early G1 cells, declining in S/G2, and absent in M phase, and has been proposed to contribute to cell cycle regulation. Because of this complex biology, the elaborate machinery regulating ciliary assembly and disassembly receives input from many cellular proteins relevant to cell cycle control, development, and oncogenic transformation, making study of gene...Continue Reading

References

Jan 1, 1986·Annual Review of Cell Biology·P A Lefebvre, J L Rosenbaum
Nov 5, 2002·Genes & Development·James K ChenPhilip A Beachy
Mar 17, 2004·The Journal of Cell Biology·Carlo IominiGianni Piperno
Nov 4, 2005·Journal of the American Society of Nephrology : JASN·Moe R MahjoubLynne M Quarmby
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Jul 3, 2007·Cell·Elena N PugachevaErica A Golemis
Feb 12, 2008·Current Protocols in Molecular Biology·Robert E KingstonJohn K Rose
Apr 9, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Lotte B PedersenSøren T Christensen
Jun 4, 2008·Journal of Biomolecular Screening·D A RossF Santini
Mar 3, 2009·The Journal of Clinical Investigation·Norann A Zaghloul, Nicholas Katsanis
Nov 20, 2009·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Giulia MantovaniGiovanni Vozzi
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andreas KnödlerWei Guo
Jan 1, 2009·Methods in Cell Biology·Sarah C GoetzKathryn V Anderson
Jan 1, 2009·Methods in Cell Biology·Muqing CaoJunmin Pan
Jun 10, 2010·The Journal of Cell Biology·Yujie LiJinghua Hu
Jun 15, 2010·Cellular and Molecular Life Sciences : CMLS·Jill A Green, Kirk Mykytyn
Feb 1, 2011·Biology of the Cell·Laetitia VincensiniPhilippe Bastin
Mar 12, 2011·Nature Cell Biology·Sehyun KimLeonidas Tsiokas
May 4, 2011·Current Opinion in Nephrology and Hypertension·Roman-Ulrich MüllerThomas Benzing
May 10, 2011·Methods in Cell Biology·Jarema MalickiZhaoxia Sun
Oct 7, 2011·Molecular Biology of the Cell·Christine E LarkinsTamara Caspary
Feb 14, 2012·Current Biology : CB·Hiroaki IshikawaWallace F Marshall
Apr 25, 2012·The Journal of Cell Biology·Akihito InokoMasaki Inagaki
May 25, 2012·Molecular Biology of the Cell·Olga V PlotnikovaErica A Golemis
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Junmin PanErica A Golemis
Jul 24, 2012·Current Opinion in Cell Biology·Tamina Seeger-Nukpezah, Erica A Golemis
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tamina Seeger-NukpezahErica A Golemis
Oct 8, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robin M Delaine-SmithGwendolen C Reilly
Jul 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·John J LeePhilip A Beachy
Aug 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anna S NikonovaErica A Golemis
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Aug 25, 2015·EMBO Reports·Kenneth Bødtker SchouSøren Tvorup Christensen
Nov 21, 2015·Developmental Cell·David U MickMaxence V Nachury
Feb 11, 2016·Oncotarget·Niamat Ali KhanJohannes V Swinnen
Mar 24, 2016·Nature·M DellingD E Clapham
Apr 19, 2016·Cell·Christopher J TapeClaus Jørgensen
Aug 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Samuel DeanKeith Gull
Oct 13, 2016·Assay and Drug Development Technologies·Romina J PaglieroDavid A Egan
Nov 20, 2016·The Journal of Pathology·Hannah M Mitchison, Enza Maria Valente

❮ Previous
Next ❯

Citations

Nov 30, 2019·Frontiers in Genetics·Noriaki SasaiToru Kondo
Mar 15, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anna A KiselevaErica A Golemis
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Marijn F StokmanAlexandre Benmerah

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
transfection
Profiler
confocal microscopy
fluorescence microscopy
Fluorescence

Software Mentioned

ImageJ
Operetta
Thermofisher HCS Studio Cell Analysis
InCELL Analyzer
Leica Application Suite Advanced Fluorescence ( LAS AF )
Thermofisher Cellinsight CX5
MetaXpress
Multiwavelength Scoring
MetaExpress
InCell Developer toolbox

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Methods in Cell Biology
Olga V PlotnikovaErica A Golemis
Nature Cell Biology
Irma Sánchez, Brian David Dynlacht
Biochemical Society Transactions
Vladislav KorobeynikovErica A Golemis
© 2022 Meta ULC. All rights reserved