Microscopy beyond the diffraction limit using actively controlled single molecules.

Journal of Microscopy
W E Moerner

Abstract

In this short review, the general principles are described for obtaining microscopic images with resolution beyond the optical diffraction limit with single molecules. Although it has been known for several decades that single-molecule emitters can blink or turn on and off, in recent work the addition of on/off control of molecular emission to maintain concentrations at very low levels in each imaging frame combined with sequential imaging of sparse subsets has enabled the reconstruction of images with resolution far below the optical diffraction limit. Single-molecule active control microscopy provides a powerful window into information about nanoscale structures that was previously unavailable.

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Citations

Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mikael P BacklundW E Moerner
Mar 1, 2014·Nature Methods·Ulrike Endesfelder, Mike Heilemann
Aug 16, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beth L HaasJulie S Biteen
Sep 19, 2012·Cellular Microbiology·Christophe Zimmer
Nov 14, 2012·EMBO Reports·Jean-Baptiste Sibarita, Mike Heilemann
Jan 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jin Wang
Sep 10, 2016·Nano Letters·Jinxing LiJoseph Wang
Sep 11, 2016·Analytical and Bioanalytical Chemistry·Bartosz TurkowydUlrike Endesfelder
Jan 14, 2017·Chemical Reviews·Katherine A WilletsPadmanabh B Joshi
Dec 18, 2013·Nature Reviews. Microbiology·Andreas Gahlmann, W E Moerner
Dec 1, 2015·Faraday Discussions·W E MoernerQuan Wang
Jan 3, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Mikael P BacklundW E Moerner
Jan 17, 2013·Physical Chemistry Chemical Physics : PCCP·Katherine A Willets
Jul 6, 2013·Angewandte Chemie·Steven F LeeAlexandre Fürstenberg
May 27, 2015·Nanotechnology·Taras PlakhotnikHuan-Cheng Chang
Nov 10, 2012·Physical Chemistry Chemical Physics : PCCP·Karole L BlytheKatherine A Willets
Aug 9, 2013·Physical Chemistry Chemical Physics : PCCP·Alexandre Fürstenberg, Mike Heilemann
Jul 25, 2014·Chemistry : a European Journal·Jaume Garcia-AmorósFrançisco M Raymo
Feb 14, 2017·Methods and Applications in Fluorescence·Navneet C VermaChayan K Nandi
Jan 1, 2016·Nature Photonics·Yoav ShechtmanW E Moerner
Dec 13, 2019·Nature Communications·James W P BrownYann Gambin
Mar 25, 2020·Biomedical Optics Express·Leonhard MöcklW E Moerner
Jun 20, 2013·Physical Chemistry Chemical Physics : PCCP·Françisco M Raymo
Dec 7, 2013·Chemical Society Reviews·Katherine A Willets
Dec 7, 2013·Chemical Society Reviews·H Peter Lu
Oct 31, 2012·Chemistry : a European Journal·Erhan DenizSalvatore Sortino
Apr 4, 2018·Optics Express·Yongzhuang ZhouAndrew R Harvey
Jun 20, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Katherine A Willets
May 23, 2013·Physical Chemistry Chemical Physics : PCCP·Sherif Shaban RagabFrançisco M Raymo
Oct 1, 2020·Computational and Structural Biotechnology Journal·Erik Meijering
Mar 9, 2021·Journal of Neuroscience Research·Patrick Jarmo PaasilaManuel B Graeber

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