Microscopy illumination engineering using a low-cost liquid crystal display

Biomedical Optics Express
Kaikai GuoGuoan Zheng

Abstract

Illumination engineering is critical for obtaining high-resolution, high-quality images in microscope settings. In a typical microscope, the condenser lens provides sample illumination that is uniform and free from glare. The associated condenser diaphragm can be manually adjusted to obtain the optimal illumination numerical aperture. In this paper, we report a programmable condenser lens for active illumination control. In our prototype setup, we used a $15 liquid crystal display as a transparent spatial light modulator and placed it at the back focal plane of the condenser lens. By setting different binary patterns on the display, we can actively control the illumination and the spatial coherence of the microscope platform. We demonstrated the use of such a simple scheme for multimodal imaging, including bright-field microscopy, darkfield microscopy, phase-contrast microscopy, polarization microscopy, 3D tomographic imaging, and super-resolution Fourier ptychographic imaging. The reported illumination engineering scheme is cost-effective and compatible with most existing platforms. It enables a turnkey solution with high flexibility for researchers in various communities. From the engineering point-of-view, the reported illum...Continue Reading

References

Aug 19, 2007·Nature Methods·Wonshik ChoiMichael S Feld
Jun 12, 2006·Optics Express·Ran YiJerome Mertz
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Guoan ZhengChanghuei Yang
Oct 18, 2011·Optics Letters·Guoan ZhengChanghuei Yang
Dec 11, 2013·Optics Letters·Xiaoze OuGuoan Zheng
Jun 19, 2014·Biomedical Optics Express·Siyuan DongGuoan Zheng
Jun 21, 2014·Journal of Biomedical Optics·Anthony WilliamsRichard Cote
Jul 30, 2014·Biomedical Optics Express·Lei TianLaura Waller
Sep 23, 2014·Nature Photonics·Guoan ZhengChanghuei Yang
Oct 17, 2014·Optics Express·Roarke HorstmeyerChanghuei Yang
Nov 2, 2014·Biomedical Optics Express·Siyuan DongGuoan Zheng

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Citations

Sep 30, 2015·Biomedical Optics Express·Kaikai GuoGuoan Zheng
May 15, 2015·Optics Express·Zichao BianGuoan Zheng
Dec 30, 2015·Biomedical Optics Express·Donghak LeeChulmin Joo
Aug 19, 2016·Applied Optics·Sanchari SenLuis Grave de Peralta
Jan 4, 2018·Journal of Biomedical Optics·Jesus Ruiz-SantaquiteriaGloria Bueno
Oct 1, 2019·Biomedical Optics Express·Sara KheireddineSebastian Wachsmann-Hogiu
Sep 6, 2018·Applied Optics·Hira FarooqLuis Grave de Peralta
Aug 2, 2018·Optics Letters·Guk-Jong ChoiYoung-Pil Park
Dec 14, 2016·Optics Express·Zichao BianGuoan Zheng
Aug 17, 2018·Optics Express·Sueli Skinner-RamosLuis Grave de Peralta
Nov 30, 2016·Biomedical Optics Express·Jaebum ChungChanghuei Yang
Jan 8, 2021·Biomedical Optics Express·Youqiang ZhuLi Xuan

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