Microspectrometric insights on the uptake of antibiotics at the single bacterial cell level

Scientific Reports
Bertrand CinquinJean-Marie Pagès

Abstract

Bacterial multidrug resistance is a significant health issue. A key challenge, particularly in Gram-negative antibacterial research, is to better understand membrane permeation of antibiotics in clinically relevant bacterial pathogens. Passing through the membrane barrier to reach the required concentration inside the bacterium is a pivotal step for most antibacterials. Spectrometric methodology has been developed to detect drugs inside bacteria and recent studies have focused on bacterial cell imaging. Ultimately, we seek to use this method to identify pharmacophoric groups which improve penetration, and therefore accumulation, of small-molecule antibiotics inside bacteria. We developed a method to quantify the time scale of antibiotic accumulation in living bacterial cells. Tunable ultraviolet excitation provided by DISCO beamline (synchrotron Soleil) combined with microscopy allows spectroscopic analysis of the antibiotic signal in individual bacterial cells. Robust controls and measurement of the crosstalk between fluorescence channels can provide real time quantification of drug. This technique represents a new method to assay drug translocation inside the cell and therefore incorporate rational drug design to impact antib...Continue Reading

References

Jan 1, 1989·Antimicrobial Agents and Chemotherapy·J S Chapman, N H Georgopapadakou
Jul 1, 1982·Journal of Biochemistry·Y Fukunaga, F Sakiyama
Feb 7, 2001·Journal of Agricultural and Food Chemistry·T J Simat, H Steinhart
Jun 11, 2002·Biochemical and Biophysical Research Communications·Monique MalléaJean-Marie Pagès
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Elizabeth Pradel, Jean-Marie Pagès
May 6, 2003·The Journal of Biological Chemistry·Diane E HeckJeffrey D Laskin
Sep 11, 2008·Current Drug Targets·Anne Davin-RegliJean-Marie Pagès
Nov 11, 2008·Nature Reviews. Microbiology·Jean-Marie PagèsMathias Winterhalter
Nov 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Helen W BoucherJohn Bartlett
Feb 24, 2009·Annual Review of Biochemistry·H Nikaido
Oct 22, 2009·Journal of Synchrotron Radiation·Alexandre GiulianiMatthieu Réfrégiers
Aug 6, 2010·Nature·Benjamin D BaxMichael N Gwynn
Aug 27, 2010·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Frédéric JammeMatthieu Réfrégiers
Oct 12, 2010·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Louis B Rice
Jun 29, 2011·FEMS Microbiology Reviews·Dan I Andersson, Diarmaid Hughes
Jun 29, 2011·FEMS Microbiology Reviews·H Nikaido, Jean-Marie Pagès
Jul 5, 2011·FEMS Microbiology Reviews·Rafael Cantón, María-Isabel Morosini
Jan 22, 2013·The Lancet Infectious Diseases·John H RexJennifer Jackson
Jan 26, 2013·The Lancet Infectious Diseases·Elizabeth M H WellingtonA Prysor Williams
Mar 23, 2013·Biology of the Cell·Frédéric JammeMatthieu Réfrégiers
Nov 21, 2013·The Lancet Infectious Diseases·Ramanan LaxminarayanOtto Cars
Jan 25, 2014·Marine Pollution Bulletin·Sergio Manzetti, Rossella Ghisi
May 7, 2014·Annals of the New York Academy of Sciences·John H RexCarrie Harney
May 28, 2014·Nature Reviews. Microbiology·Dan I Andersson, Diarmaid Hughes
Mar 4, 2015·Antimicrobial Agents and Chemotherapy·Ozden Kocaoglu, Erin E Carlson
Jun 4, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mathias Winterhalter, Matteo Ceccarelli

Citations

Oct 21, 2016·Expert Opinion on Drug Metabolism & Toxicology·Sandrine AlibertJean-Marie Pagès
Jul 19, 2019·Clinical Microbiology Reviews·Anne Davin-RegliJean-Marie Pagès
Aug 31, 2017·Scientific Reports·Julia VergalliJean-Marie Pagès
Apr 22, 2017·Scientific Reports·Anas AllamJean-Marie Pagès
Feb 23, 2017·Nature Microbiology·Muriel MasiJean-Marie Pagès
Mar 16, 2018·Clinical Microbiology Reviews·Ronald DomalaonFrank Schweizer
Jul 16, 2019·MedChemComm·M Rhia L StoneMark A T Blaskovich
May 9, 2020·Journal of Synchrotron Radiation·Frédéric JammeMarine Froissard
Feb 5, 2020·International Journal of Molecular Sciences·Alessio AtzoriPaolo Ruggerone
Jan 9, 2019·Life Science Alliance·Estelle DumontJean-Marie Pagès
Jun 6, 2019·Annals of the New York Academy of Sciences·Helen I Zgurskaya, Valentin V Rybenkov
Nov 10, 2020·Expert Opinion on Drug Delivery·Mathias Winterhalter

Related Concepts

Bacteriocidal Agents
Oxidation-Reduction
Microspectrophotometry
Reactive Oxygen Species
Antibiotic Resistance, Bacterial
Single-Cell Analysis
Antibiotics
Gram-Negative Bacteria
Ion Channel
Microscopy

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.