Abstract
Bacterial cellulose (BC)⁻gelatin (GEL) membranes were processed by successive periodate oxidation and a freeze-thawing/carbodiimide crosslinking procedure, first facilitating a Schiff-base reaction among respective aldehyde and hydroxyl groups, and later GEL stabilization and microstructuring. The formation of highly microporous structures within the GEL portion, with significant differences between bottom and top, was elucidated, and pores in the 27.6 ± 3 µm⁻108 ± 5 µm range were generated, exceeding the threshold value of ~10 µm sufficient for cell trafficking. During a relatively short (6 h) exhaustion procedure in supersaturated simulated body fluid solution, the membranes accommodated the combination of biologically relevant minerals, i.e., flake-like octacalcium phosphate (OCP) and (amorphous) apatite, onto their surface, forming a membrane with intensive swelling (650⁻1650%) and up to 90% weight loss in a 4-week period. The membranes´ 6-day eluates did not evoke any cytotoxic effects toward human fibroblast, MRC-5 cells. The same type of cells retained their morphology in direct contact with the membrane, attaching to the GEL porous site, while not attaching to the GEL thin-coated BC side, most probably due to combined, ...Continue Reading
References
Nov 1, 1988·Biopolymers·K J Payne, A Veis
Feb 21, 1998·Advances in Dental Research·A B Sønju Clasen, I E Ruyter
Jul 12, 2005·Biomacromolecules·S DawleeA Jayakrishnan
May 9, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·L E Millon, W K Wan
May 23, 2006·Biomaterials·Stacy A HutchensClaudia J Rawn
Dec 31, 2009·Acta Biomaterialia·E BoaniniA Bigi
Jun 18, 2011·Journal of Biomedical Materials Research. Part a·Fábia K AndradeMiguel Gama
Aug 9, 2011·Biotechnology Advances·Lucie BacakovaVaclav Svorcik
Jan 30, 2013·Biochimica Et Biophysica Acta·Lisa M MillerRandy J Smith
Apr 3, 2013·Carbohydrate Polymers·Wen-Chun LinShan-Hui Hsu
Dec 19, 2013·Biomaterials·Sandeep T KoshyDavid J Mooney
Jan 7, 2014·Journal of Biomedical Materials Research. Part a·Selestina GorgievaVanja Kokol
Jan 25, 2014·Colloids and Surfaces. B, Biointerfaces·Diosángeles Soto VélizParvez Alam
Apr 18, 2014·Biomedical Materials·Jian WuZhigu Wu
Jun 14, 2014·Journal of Biomedical Materials Research. Part a·Selestina Gorgieva, Vanja Kokol
Apr 10, 2015·PloS One·Sabra I DjomehriSunita P Ho
Apr 22, 2015·The Open Biomedical Engineering Journal·X LinY Liu
May 15, 2015·Acta Biomaterialia·Saad B QasimIhtesham Ur Rehman
May 26, 2015·Carbohydrate Polymers·H G Oliveira BarudSidney J L Ribeiro
Sep 24, 2015·Biotechnology Journal·Mazhar Ul-IslamJoong Kon Park
Aug 23, 2016·Scientific Reports·Shiqing MaPing Gao
Sep 2, 2016·Journal of Materials Science. Materials in Medicine·Natalia DavidenkoRuth E Cameron
Jul 25, 2017·Biotechnology Reports·W TreesuppharatS Ummartyotin
Aug 5, 2017·Materials·Patricia NeacsuAnisoara Cimpean
Oct 21, 2017·Carbohydrate Polymers·Wei ShaoXiufeng Liu
Jan 22, 2018·Carbohydrate Polymers·Hui QiaoYajie Xie
Apr 21, 2018·ACS Applied Materials & Interfaces·Tina TronserPavel A Levkin
May 4, 2018·ACS Central Science·Atsushi SakaiMiho Yanagisawa
Sep 13, 2018·ACS Applied Materials & Interfaces·Junchuan YangXingyu Jiang
Oct 3, 2018·Materials Science & Engineering. C, Materials for Biological Applications·Selestina GorgievaVanja Kokol
Apr 11, 2019·Polymers·Jing WangYouhong Tang
May 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pedro S BaboManuela E Gomes
Aug 3, 2017·Engineering in Life Sciences·Ji Eun SongCarla Silva
Citations
Sep 10, 2020·Journal of Biomaterials Science. Polymer Edition·Chhavi SharmaPuneet Pathak
Sep 25, 2019·Nanomaterials·Selestina Gorgieva, Janja Trček
Apr 3, 2020·Nanomaterials·Laura MalešSelestina Gorgieva
Aug 9, 2021·Carbohydrate Polymers·Xucai WangCaoxing Huang
May 24, 2019·Biomacromolecules·Ding WangChan Beum Park
Aug 29, 2021·Pharmaceutics·Raluca NicuDiana E Ciolacu