Microstructured and Degradable Bacterial Cellulose⁻Gelatin Composite Membranes: Mineralization Aspects and Biomedical Relevance

Nanomaterials
Selestina Gorgieva, Silvo Hribernik

Abstract

Bacterial cellulose (BC)⁻gelatin (GEL) membranes were processed by successive periodate oxidation and a freeze-thawing/carbodiimide crosslinking procedure, first facilitating a Schiff-base reaction among respective aldehyde and hydroxyl groups, and later GEL stabilization and microstructuring. The formation of highly microporous structures within the GEL portion, with significant differences between bottom and top, was elucidated, and pores in the 27.6 ± 3 µm⁻108 ± 5 µm range were generated, exceeding the threshold value of ~10 µm sufficient for cell trafficking. During a relatively short (6 h) exhaustion procedure in supersaturated simulated body fluid solution, the membranes accommodated the combination of biologically relevant minerals, i.e., flake-like octacalcium phosphate (OCP) and (amorphous) apatite, onto their surface, forming a membrane with intensive swelling (650⁻1650%) and up to 90% weight loss in a 4-week period. The membranes´ 6-day eluates did not evoke any cytotoxic effects toward human fibroblast, MRC-5 cells. The same type of cells retained their morphology in direct contact with the membrane, attaching to the GEL porous site, while not attaching to the GEL thin-coated BC side, most probably due to combined, ...Continue Reading

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Citations

Sep 25, 2019·Nanomaterials·Selestina Gorgieva, Janja Trček

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Methods Mentioned

BETA
X-Ray
Fluorescence
fluorescence microscopy
Assay

Software Mentioned

ImageJ
BoneJ
SPSS
LAS AF
Spectrum

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