PMID: 19146762Jan 17, 2009Paper

Microsurgical cloning techniques for zygotes: a long-term dispute

Reproductive Biomedicine Online
Karl Illmensee

Abstract

Zygotes (fertilized oocytes) have been considerably neglected as suitable recipients for cell nuclear transfer in cloning. Recently, it has been reported by Polish researchers that microsurgical methods using very thin pipettes for pronuclear removal from mouse zygotes seem to be crucial for successful cloning of adult mice. It was postulated that due to this technique both pronuclei are broken, leaving behind some nuclear components in the oocyte's cytoplasm. Release of pronuclear factors into the cytoplasm turns enucleated zygotes into suitable nuclear recipients for cloning.

References

Dec 1, 1989·Die Naturwissenschaften·K IllmenseeJ A Modlinski
Feb 2, 2000·Nature Genetics·T WakayamaR Yanagimachi
Oct 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Pawel GredaJacek A Modlinski
Nov 28, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Anita SchurmannBjörn Oback

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Citations

Jan 22, 2015·Orthopaedic Nursing·Erden Ali, Vikas Khanduja

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