Microsystem for transfection of exogenous molecules with spatio-temporal control into adherent cells

Biosensors & Bioelectronics
Tilak Jain, Jit Muthuswamy

Abstract

Several non-viral techniques involving the use of liposomes, particle bombardment and electroporation have been used for efficient transfection of plasmids and other molecules into cells. Current approaches target whole or bulk regions of tissue, lacking the desired spatial control over the transfection process. In this study, we present a novel approach using microsystems to achieve spatial and temporal control over the transfection process in adherent cells. A 6x6 MEA (microelectrode array) with 100 microm microelectrode dimension was developed on a silicon substrate using standard microfabrication procedures and passivated with a biocompatible layer. Using finite element models, electric field intensities were simulated and locations of optimal electroporation zones in the cell culture on the microelectrode surface were predicted. The MEA was subsequently tested using 3T3 fibroblasts cultured on the MEA surface for 96 h and stimulation voltages in the range of 2-5 V in the presence of propidium iodide (PI), a cell impermeant dye. Maximum electric field intensities in the z-direction were estimated to be in the range of 320-820 V/cm for applied differential voltages in the range of 2-5 V. Cells directly on the top and on the ...Continue Reading

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Citations

Mar 28, 2013·Molecular Therapy. Nucleic Acids·Arati SridharanJit Muthuswamy
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Won Gu LeeAli Khademhosseini
Aug 7, 2013·Lab on a Chip·Tao Geng, Chang Lu
Jun 6, 2009·Biosensors & Bioelectronics·Ching-Wen HuangTai-Horng Young
Apr 19, 2008·Journal of Neuroscience Methods·Nathan Jackson, Jit Muthuswamy
Feb 14, 2008·IEEE Transactions on Bio-medical Engineering·Tilak Jain, Jit Muthuswamy

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