Microtome-free 3-dimensional confocal imaging method for visualization of mouse intestine with subcellular-level resolution.

Gastroenterology
Ya-Yuan FuShiue-Cheng Tang

Abstract

The intrinsic opacity of mouse intestinal tissue prevents its evaluation by high-resolution, in-depth optical microscopy. Instead, intestinal tissue is usually sectioned to expose the interior domains of the mucosa and submucosa for microscopic examination. However, microtome sectioning can cause distortions and artifacts that prevent acquisition of an accurate view of the sample. We therefore attempted to develop a microtome-free 3-dimensional (3D) confocal imaging method for characterization of mouse intestine. We applied an optical-clearing solution, FocusClear, to permeate and reduce the opacity of mouse colon and ileum. Tissues were labeled with fluorescent probes and examined by confocal microscopy with efficient fluorescence excitation and emission in the FocusClear solution. The voxel-based confocal micrographs were processed with Amira software for 3D visualization and analysis. Treatment of tissues with the optical-clearing solution improved photon penetration, resulting in the acquisition of images with subcellular-level resolution across the mucosa, submucosa, and muscle layers. Collectively, the acquired image stacks were processed by projection algorithms for 3D analysis of the spatial relations in villi, crypts, ...Continue Reading

References

Dec 1, 1994·Current Opinion in Cell Biology·J I Gordon, M L Hermiston
Apr 16, 2003·Methods : a Companion to Methods in Enzymology·Yee-Chien Liu, Ann-Shyn Chiang
Jan 20, 2004·The Journal of Comparative Neurology·John L MignoneGrigori Enikolopov
Mar 9, 2004·Lasers in Surgery and Medicine·Misbah H KhanJ Stuart Nelson
Aug 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki AmohRobert M Hoffman
Sep 14, 2004·Gastroenterology·Michael H Nathanson
Jan 25, 2005·Lasers in Surgery and Medicine·Bernard ChoiJ Stuart Nelson
Mar 30, 2005·Archives of Pathology & Laboratory Medicine·Mark Li-cheng WuBela Molnar
Apr 13, 2005·Current Biology : CB·Shouzhen XiaAnn-Shyn Chiang
Jun 9, 2005·Gastroenterology·Ralf KiesslichMarkus F Neurath
Aug 13, 2005·Microvascular Research·Dino J RavnicMoritz A Konerding
Sep 7, 2005·Gastroenterology·Carol R Regueiro, UNKNOWN AGA Future Trends Committee
Nov 22, 2005·Nature Methods·José-Angel Conchello, Jeff W Lichtman
Nov 22, 2005·Nature Methods·Fritjof Helmchen, Winfried Denk
Mar 27, 2007·Cell·Hui-Hao LinAnn-Shyn Chiang
Apr 5, 2007·Nature Protocols·Stefan WirtzMarkus F Neurath
Apr 10, 2007·The Journal of Surgical Research·Katsuhiro HayashiMichael Bouvet
Jul 28, 2007·Nature Clinical Practice. Oncology·Ralf KiesslichMarkus F Neurath
Dec 7, 2007·Gastroenterology·Ralf KiesslichAlastair J M Watson
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yalin WangYi Zhong

❮ Previous
Next ❯

Citations

Mar 17, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuan-An LiuShiue-Cheng Tang
Jul 9, 2013·Archives of Pathology & Laboratory Medicine·Richard TorresMichael J Levene
Sep 10, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Si Hyun KimJong Hwan Sung
Nov 6, 2010·Expert Review of Medical Devices·Elina A GeninaValery V Tuchin
Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Aug 24, 2010·Gastroenterology·Ya-Yuan Fu, Shiue-Cheng Tang
Oct 28, 2015·Biomedical Optics Express·Eunjoo SongPilhan Kim
Nov 13, 2013·Optics Express·Alicia ArranzJorge Ripoll
Oct 27, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H-J ChienS-C Tang
Mar 19, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y A LiuS C Tang
Sep 8, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y-A LiuS-C Tang
Nov 16, 2011·IEEE Transactions on Bio-medical Engineering·Hao-Chiang ShaoYung-Chang Chen
Apr 30, 2016·Biotechnology Journal·Seung Hwan LeeJong Hwan Sung
May 5, 2016·Progress in Histochemistry and Cytochemistry·Adriano AzaripourCornelis J F Van Noorden
Jun 25, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pei-Yu LinShiue-Cheng Tang
Feb 15, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Gleison D P BossolaniJean-Pierre Timmermans
Jun 27, 2013·Diabetologia·Shiue-Cheng TangYa-Yuan Fu
Jul 10, 2019·Biotechnology and Bioengineering·Elisabete C CostaIlídio J Correia
Jan 16, 2014·American Journal of Physiology. Endocrinology and Metabolism·Jyuhn-Huarng JuangShiue-Cheng Tang
Feb 28, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Cambrian Y LiuD Brent Polk
May 19, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Cambrian Y Liu, D Brent Polk
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hung-Jen ChienShiue-Cheng Tang
Jan 1, 2019·Current Pathobiology Reports·Yang Liu, Jianquan Xu
Oct 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ya-Yuan FuShiue-Cheng Tang
Feb 4, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tiantian GuoBin Feng
Feb 17, 2021·Methods and Applications in Fluorescence·Sergiy V Avilov
Aug 1, 2021·Nature Reviews. Cancer·Jorge AlmagroAxel Behrens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.