Microtomographic analysis of neuronal circuits of human brain

Cerebral Cortex
Ryuta MizutaniYoshio Suzuki

Abstract

We report a 3D analysis of the neuronal circuits of human cerebral cortex. Neuronal circuits, which are essential for brain functions, are built up by neurons as a 3D network, so tracing the 3D neuronal network of human cerebral cortex is the first step to understanding the mechanism of human brain functions. The cortical microstructures were visualized by X-ray microtomographic imaging of adult frontal cortex tissue stained with metal impregnation. Skeletonized wire models were built by tracing the 3D distribution of X-ray absorption coefficients. The obtained neuronal models were composed of 240 pyramidal neurons and 131 interneurons. Capillary vessel structures along with blood cells in the capillary lumen were also visualized and traced to build capillary network models. Possible neuronal circuits were analytically resolved from the skeletonized wire models. The operating mechanism of the resolved circuits is discussed on the basis of neurotransmission in the circuits. The results also indicate that X-ray microtomography is a potential method of visualizing the neuronal circuits of the brain.

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Citations

Nov 1, 2011·Micron : the International Research and Review Journal for Microscopy·Ryuta Mizutani, Yoshio Suzuki
Jul 22, 2014·Scientific Reports·Ryuta MizutaniYoshio Suzuki
Sep 10, 2013·Journal of Structural Biology·Ryuta MizutaniYoshio Suzuki
Oct 12, 2016·Scientific Reports·Ryuta MizutaniKentaro Uesugi
Dec 25, 2013·Journal of Synchrotron Radiation·Elisabeth SchültkeGuido Nikkhah
Jul 6, 2019·Journal of Synchrotron Radiation·Lorella PascoloGiuseppe Ricci
Jun 15, 2013·Journal of Synchrotron Radiation·Ryuta MizutaniYoshio Suzuki
Feb 14, 2019·Translational Psychiatry·Ryuta MizutaniMakoto Arai
Dec 15, 2020·Experimental Eye Research·Sergey Y TkachevMarina S Tkacheva
Jan 16, 2021·Translational Psychiatry·Ryuta MizutaniMakoto Arai
Apr 6, 2021·Frontiers in Neuroscience·Paulla Vieira RodriguesMatheus de Castro Fonseca
Jul 6, 2019·ACS Chemical Neuroscience·Damien DepannemaeckerJean Rinkel

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