Microtox toxicity test: detoxification of TNT and RDX contaminated solutions by poplar tissue cultures

Chemosphere
Brittany R FlokstraJerald L Schnoor

Abstract

Poplar (Populus deltoidesxnigra DN34) tissue cultures removed 2,4,6-trinitrotoluene (TNT) from an aqueous solution in five days, reducing the toxicity of the solution from highly toxic Microtox EC value to that of the control. 1,3,5-Trinitro-1,3,5-triazacyclohexane (RDX) was taken up by the plant tissue cultures more slowly, but toxicity reduction of the solution was evident. The measurement of toxicity reduction of aqueous solutions containing TNT and RDX was performed using a novel methodology developed for use with the Microtox testing system. Radiolabeled TNT and RDX were used to confirm removal of explosives from hydroponic solutions containing plant tissue cultures and to verify that toxicity did not change in solutions where no plant cultures were present (positive controls). High Performance Liquid Chromatography (HPLC) and Liquid Scintillation Counter (LSC) measurements confirmed removal of TNT and RDX from solutions containing poplar plant tissue cultures and constancy of the plant-free controls. In addition, metabolites were identified in remediated solutions by HPLC, confirming the mechanism by which plants can remediate groundwater, surface water, and soil solutions.

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Citations

Apr 28, 2009·Journal of Hazardous Materials·Marcio Barreto-RodriguesTeresa C B Paiva
Sep 27, 2008·Journal of Hazardous Materials·Marcio Barreto-RodriguesTeresa C B Paiva
Sep 4, 2013·The Science of the Total Environment·Xiaoyan Y MaNa Wang
May 12, 2009·Environmental Pollution·S Sagi-Ben MosheR Nativ
Apr 2, 2015·Journal of Hazardous Materials·Raquel LudwichkMarcio Barreto-Rodrigues
Feb 11, 2010·Journal of Environmental Monitoring : JEM·Konstantinos C MakrisRupali Datta
Jan 18, 2018·Environmental Science and Pollution Research International·Werther Guidi NissimElisa Azzarello
May 8, 2020·Environmental Science and Pollution Research International·Ali B Abou HammadAkebe Luther King Abia
Feb 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tamer El MalahWael A El-Sayed
Feb 8, 2019·Environmental Science and Pollution Research International·Bahaa Ahmed HemdanAli Belal Abou Hammad
Oct 23, 2008·Environmental Science & Technology·Pieter Van DillewijnJuan L Ramos

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