Microtubule alignment and manipulation using AC electrokinetics

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Maruti UppalapatiWilliam O Hancock

Abstract

The kinesin-microtubule system plays an important role in intracellular transport and is a model system for integrating biomotor-driven transport into microengineered devices. AC electrokinetics provides a novel tool for manipulating and organizing microtubules in solution, enabling new experimental geometries for investigating and controlling the interactions of microtubules and microtubule motors in vitro. By fabricating microelectrodes on glass substrates and generating AC electric fields across solutions of microtubules in low-ionic-strength buffers, bundles of microtubules are collected and aligned and the electrical properties of microtubules in solution are measured. The AC electric fields result in electro-osmotic flow, electrothermal flow, and dielectrophoresis of microtubules, which can be controlled by varying the solution conductivity, AC frequency, and electrode geometry. By mapping the solution conductivity and AC frequency over which positive dielectrophoresis occurs, the apparent conductivity of taxol-stabilized bovine-brain microtubules in PIPES buffer is measured to be 250 mS m(-1). By maximizing dielectrophoretic forces and minimizing electro-osmotic and electrothermal flow, microtubules are assembled into op...Continue Reading

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Citations

Feb 18, 2011·Applied Microbiology and Biotechnology·Jennelle L Malcos, William O Hancock
Jun 8, 2010·Nature Nanotechnology·Erik M FreerDavid P Stumbo
Jun 10, 2009·Physical Chemistry Chemical Physics : PCCP·Mark E ArsenaultYale E Goldman
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Jan 19, 2016·Small·Eva-Maria LauxRalph Hölzel
Feb 13, 2013·Electrophoresis·Asuka Nakano, Alexandra Ros
Oct 30, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Maruti UppalapatiWilliam O Hancock
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