PMID: 3768299Aug 12, 1986Paper

Microtubule assembly is dependent on a cluster of basic residues in alpha-tubulin

Biochemistry
J SzaszH Sternlicht

Abstract

Previous studies have shown that tubulin, a major protein component of the microtubule, is rendered assembly incompetent when a highly reactive lysine residue (HRL) in the alpha polypeptide of tubulin dimer is reductively methylated [cf. Sherman, G., Rosenberry, T. L., & Sternlicht, H. (1983) J. Biol. Chem. 258, 2148-2156]. In this study we demonstrate that the HRL in bovine brain tubulin is Lys-394, a residue proximal in the alpha-tubulin sequence to the highly negatively charged carboxy-terminus region (residues 412-450) previously implicated in assembly. pH studies were undertaken to probe the local environment of Lys-394. These studies indicated that Lys-394 reactivity toward HCHO is sensitive to the titration of a pKa 6.3 group presumed to be a histidine residue. This assignment is supported by our finding that histidine modification via diethyl pyrocarbonate strongly affects Lys-394 reactivity toward HCHO as well as microtubule assembly. We propose on the basis of secondary structure considerations and published sequence data for a variety of tubulins that Lys-394 is part of an evolutionarily conserved cluster of basic residues (effective charge: 2+ to 2.5+ at neutral pH) composed of Lys-394, His-393, and Arg-390, which i...Continue Reading

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Citations

May 26, 1999·Cell Motility and the Cytoskeleton·L KniplingJ Wolff
Jan 1, 1991·Cell Motility and the Cytoskeleton·R G Burns
Nov 6, 2009·Genes & Nutrition·Blythe D ShepardPamela L Tuma
Jun 1, 1990·Prostaglandins·K K MurthiH Sternlicht
May 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·J W WinkelmanS Kimel
Mar 25, 2011·Biomacromolecules·Ken-Ichi SanoYoshihito Osada
Jul 17, 2007·Chemical Research in Toxicology·Benjamin J StewartDennis R Petersen
May 4, 2000·Molecular Biology of the Cell·K L RichardsD Botstein
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Sep 24, 2015·Biomolecules·Jennifer L Groebner, Pamela L Tuma
Jan 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R B JennettD J Tuma
Mar 22, 1988·Biochemistry·M B YaffeH Sternlicht
Aug 28, 2014·Biomacromolecules·Nathan F Bouxsein, George D Bachand
Jul 6, 2017·International Journal of Molecular Sciences·Amelia L ParkerMaria Kavallaris
Jan 23, 2021·Current Biology : CB·Takashi HottaRyoma Ohi
Aug 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Kiyotaka ShigeharaKazuhiro Shikinaka

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