Microtubule associated motor proteins of Plasmodium falciparum merozoites

Molecular and Biochemical Parasitology
R E FowlerG H Mitchell

Abstract

We have studied the occurrence, stage specificity and cellular location of key molecules associated with microtubules in Plasmodium falciparum merozoites. Antibodies to gamma tubulin, conventional kinesin and cytoplasmic dynein were used to determine the polarity of merozoite microtubules (mt), the stage specificity of the motor proteins and their location during merozoite development. We conclude that the minus ends of the mts are located at their apical pole. Kinesin was present throughout the lifecycle, appearing as a distinct crescent at the apex of developing merozoites. The vast majority of cytoplasmic dynein reactivity occurred in late merogony, also appearing at the merozoite apex. Destruction of mt with dinitroanilines did not affect the cellular location of kinesin or dynein. In invasion assays, dynein inhibitors reduced the number of ring stage parasites. Our results show that both conventional kinesin and cytoplasmic dynein are abundant, located at the negative pole of the merozoite mt and, intriguingly, appear there only in very late merogony, prior to merozoite release and invasion.

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Citations

Apr 28, 2010·The Journal of Biological Chemistry·Wassim DaherJamal Khalife
Jun 15, 2007·The Journal of Experimental Medicine·Marek CyrklaffFriedrich Frischknecht
Mar 26, 2014·Journal of Proteomics·Carmen T Gómez de LeónRicardo Mondragón Flores
May 1, 2008·Expert Opinion on Drug Discovery·Bj FennellA Bell
May 11, 2020·Cellular Microbiology·Anat FlorentinVasant Muralidharan
Apr 11, 2003·The Journal of Biological Chemistry·Steven A HowellMichael J Blackman
May 10, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Elijah K GithuiAndrew G McArthur
Sep 19, 2021·Life Science Alliance·Caroline S SimonJulien Guizetti

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