PMID: 2484862Jan 1, 1989Paper

Microtubule-associated protein 1A (MAP 1A) is a ganglion cell marker in adult rat retina

Visual Neuroscience
L McKerracherA J Aguayo

Abstract

We have used antibodies raised against a cytoskeletal protein, microtubule-associated protein 1A (MAP 1A), to stain adult rat retina. In cryostat and polyethylene glycol-embedded radial sections, the fiber layer, ganglion cell layer, and inner plexiform layer were highly immunoreactive, a finding that suggested that the ganglion cell somata, axons, and dendrites were recognized by these antibodies. Retrograde labeling of retinal cell somata from the superior colliculus and dorso-lateral geniculate nucleus to identify ganglion cells showed colocalization of the tracer and immunoreactive cells. Double labeling with nuclear stains revealed that many cells in the ganglion cell layer, which are likely displaced amacrine cells, were not recognized by these antibodies. Furthermore, transection of ganglion cell axons, a procedure that causes retrograde degeneration of many of the axotomized ganglion cells, led to a decrease in the number of anti-MAP 1A immunoreactive cells in retinal wholemounts. Thus, MAP 1A antibodies preferentially stain ganglion cell somata and dendrites but not amacrine cells. These antibodies should be useful ganglion cell markers.

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Citations

Sep 29, 2011·Progress in Retinal and Eye Research·Manuel Vidal-SanzMaria P Villegas-Pérez
Aug 9, 2016·Molecular and Cellular Neurosciences·Marina Castro ZalisUlrica Englund Johansson
Apr 30, 2008·Visual Neuroscience·Anthony A VuglerGlen Jeffery
Jun 23, 2019·International Journal of Molecular Sciences·Beatriz Vidal-VillegasManuel Vidal-Sanz
Oct 1, 1990·Trends in Pharmacological Sciences·B Demeneix
Oct 1, 1990·Trends in Neurosciences·A M Thomson

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