Microtubule-associated protein 1b is required for shaping the neural tube

Neural Development
Pradeepa JayachandranRachel Brewster

Abstract

Shaping of the neural tube, the precursor of the brain and spinal cord, involves narrowing and elongation of the neural tissue, concomitantly with other morphogenetic changes that contribue to this process. In zebrafish, medial displacement of neural cells (neural convergence or NC), which drives the infolding and narrowing of the neural ectoderm, is mediated by polarized migration and cell elongation towards the dorsal midline. Failure to undergo proper NC results in severe neural tube defects, yet the molecular underpinnings of this process remain poorly understood. We investigated here the role of the microtubule (MT) cytoskeleton in mediating NC in zebrafish embryos using the MT destabilizing and hyperstabilizing drugs nocodazole and paclitaxel respectively. We found that MTs undergo major changes in organization and stability during neurulation and are required for the timely completion of NC by promoting cell elongation and polarity. We next examined the role of Microtubule-associated protein 1B (Map1b), previously shown to promote MT dynamicity in axons. map1b is expressed earlier than previously reported, in the developing neural tube and underlying mesoderm. Loss of Map1b function using morpholinos (MOs) or δMap1b (enc...Continue Reading

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Citations

May 9, 2018·PLoS Genetics·Erin L HeinzenUNKNOWN Epilepsy Phenome/Genome Project
Sep 6, 2017·Development·Elizabeth Carroll DriverMatthew W Kelley
Oct 20, 2018·Development·Mike R VisetsoukJennifer H Gutzman
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Oct 24, 2020·Current Opinion in Neurobiology·Fiona Francis, Silvia Cappello
Jun 3, 2021·Cells·Anke Vermehren-SchmaedickMichael S Cohen

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Methods Mentioned

BETA
neurulation
PCR

Software Mentioned

Adobe PhotoShop
Genewiz
Volocity
Metamorph
excel
Mathematica
ImageJ
Image J (
GeneTools
GraphPad

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