Microtubule associated protein 9 inhibits liver tumorigenesis by suppressing ERCC3

EBioMedicine
Jing ZhangJun Yu

Abstract

Chromosomal instability plays an important part in cancer, but its genetic basis in liver tumorigenesis remains largely unclear. We aimed to characterize the mechanistic significance and clinical implication of mitotic regulator microtubule-associated protein 9 (MAP9) in hepatocellular carcinoma (HCC). The biological functions of MAP9 were determined by in vitro tumorigenicity assays. Systematic MAP9 knockout mouse (MAP9∆/∆) and hepatocyte-specific MAP9 knockout mouse (MAP9∆/∆hep) were generated to confirm the role of MAP9 in HCC. The clinical impact of MAP9 was assessed in primary HCC tissue samples. We found that MAP9 was frequently silenced in HCC tissue samples. The transcriptional silence of MAP9 in liver cancer cell lines and tissue samples was mediated by its promoter hypermethylation. MAP9 promoter hypermethylation or downregulation was associated with poor survival and recurrence in patients with HCC. Mechanistically, ectopic expression of MAP9 in LO2 and HepG2 cell lines impaired cell proliferation, colony formation, migration and invasion, and induced cell apoptosis and cycle arrest, whereas knockdown of MAP9 in Miha cell line showed the opposite effects. We found that MAP9∆/∆ mice spontaneously developed a liver hyp...Continue Reading

Methods Mentioned

BETA
acetylation
surgical resection
PCR
protein assay
electrophoresis
transfections
transfection
flow cytometry
biopsies
genotyping

Software Mentioned

GraphPad
NES
Statistical Package for the Social Sciences ( SPSS )
GraphPad Prism
ModFitLT
SeqScape
GSEA
Gene Set Enrichment Analysis ( GSEA )

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