Dec 13, 2018

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis

Nature Communications
Hai-Qing TuHui-Yan Li

Abstract

Defective ciliogenesis causes human developmental diseases termed ciliopathies. Microtubule (MT) asters originating from centrosomes in mitosis ensure the fidelity of cell division by positioning the spindle apparatus. However, the function of microtubule asters in interphase remains largely unknown. Here, we reveal an essential role of MT asters in transition zone (TZ) assembly during ciliogenesis. We demonstrate that the centrosome protein FSD1, whose biological function is largely unknown, anchors MT asters to interphase centrosomes by binding to microtubules. FSD1 knockdown causes defective ciliogenesis and affects embryonic development in vertebrates. We further show that disruption of MT aster anchorage by depleting FSD1 or other known anchoring proteins delocalizes the TZ assembly factor Cep290 from centriolar satellites, and causes TZ assembly defects. Thus, our study establishes FSD1 as a MT aster anchorage protein and reveals an important function of MT asters anchored by FSD1 in TZ assembly during ciliogenesis.

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Mentioned in this Paper

Vertebrates
Embryo
Zone
Gene Knockdown Techniques
FSD1
Cep290 protein, mouse
Cell Division
Satellite, Centriolar
Axoneme Assembly
Spindle (Cell Division)

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