Microtubule binding of the drosophila DMAP-85 protein is regulated by phosphorylation in vitro

FEBS Letters
V CambiazoC E Sunkel

Abstract

The phosphorylation of microtubule-associated proteins (MAPs) is thought to be a key factor in the regulation of microtubule (MT) stability. Previously we isolated DMAP-85, a Drosophila MAP shown to be associated with stable MTs. In this work we show that DMAP-85 phosphorylated in cell-free early embryo extracts is released from MTs. MPM-2 antibodies recognize the phosphorylated protein. In vitro, DMAP-85 can be phosphorylated by the mitotic kinase Polo affecting its binding to MTs and creating MPM-2 epitopes on the protein. The results suggest that phosphorylation of DMAP-85 might affect its MT stabilizing activity during early mitotic cycles.

Citations

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M D WeingartenM W Kirschner
Dec 1, 1991·Genes & Development·S LlamazaresC E Sunkel
Jan 1, 1990·Annual Review of Cell Biology·E A Fyrberg, L S Goldstein
Jan 1, 1988·Cell Motility and the Cytoskeleton·D B EngleN R Morris
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F M DavisP N Rao
Oct 1, 1995·Physiological Reviews·R B Maccioni, V Cambiazo
Nov 1, 1995·The Journal of Cell Biology·D Masson, T E Kreis
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S TaageperaG J Gorbsky
Nov 1, 1993·The Journal of Cell Biology·J Kuang, C L Ashorn
Feb 1, 1995·Current Opinion in Cell Biology·E M Mandelkow, E M Mandelkow
Mar 1, 1995·Journal of Neurochemistry·V CambiazoR B Maccioni
Jan 1, 1993·Annual Review of Cell Biology·E D Schejter, E Wieschaus
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M WestendorfL Gerace
Dec 1, 1995·Molecular Biology of the Cell·B TrinczekE M Mandelkow
Feb 1, 1996·Current Opinion in Cell Biology·F J McNally
Jan 1, 1996·Cell Motility and the Cytoskeleton·E Shelden, P Wadsworth
Nov 14, 1997·The Journal of Biological Chemistry·B OuyangWei Dai
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Desai, T J Mitchison
Oct 3, 1998·Trends in Biochemical Sciences·G DrewesE M Mandelkow
Feb 27, 1999·Current Opinion in Cell Biology·L Cassimeris

Related Concepts

Microcystin
Embryo
Monoclonal Antibodies
Microcystins
Cyclic Peptides
Microtubule-Associated Proteins
Microtubule-Associated Protein 3
Entire Embryo
Drosophila melanogaster Proteins
Proteins, Recombinant DNA

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