Microtubule dependence of chromosome cycles in Xenopus laevis blastomeres under the influence of a DNA synthesis inhibitor, aphidicolin

Developmental Biology
P Clute, Y Masui

Abstract

The spindle-assembly checkpoint of the cell cycle develops in Xenopus laevis embryos at the midblastula transition (MBT). Our previous experiments using animal-cap blastomeres indicate that the checkpoint is regulated by a mechanism that depends on age, but not on the nucleocytoplasmic (N/C) ratio (Clute and Masui, 1995). In the present study, the time of appearance of the spindle-assembly checkpoint is examined in animal-cap blastomeres whose N/C ratio is reduced by treatment with aphidicolin. Animal-cap blastomeres treated with aphidicolin from the 2-cell stage cleave more slowly after 4th cleavage, in a dose-dependent manner, but cleavage and chromosome cycles continue up to the 11th to 13th cleavage and then arrest. Blastomeres treated with aphidicolin have a reduced DNA content and N/C ratio compared to control blastomeres of the same age. Nevertheless, nocodazole-sensitive chromosome cycles appear at the same time as in control blastomeres, at 3 to 5 hr after 5th cleavage, regardless of the N/C ratio. The arrest in interphase caused by treating blastula stage animals caps with aphidicolin can be reversed by treatment with caffeine. The caffeine-induced mitosis becomes sensitive to nocodazole after the MBT, but not before....Continue Reading

References

Aug 9, 1991·Cell·R Li, A W Murray
Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·H SeinoT Nishimoto
Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·R T SchimkeS W Sherwood
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A L KungR T Schimke
Jan 1, 1989·Journal of Cell Science. Supplement·J Newport, M Dasso
Jan 1, 1989·Journal of Cell Science. Supplement·A W Murray
Nov 3, 1989·Science·A W Murray, M W Kirschner
Nov 3, 1989·Science·L H Hartwell, T A Weinert
Jun 1, 1986·The Journal of Experimental Zoology·G SluderK Spanjian
Dec 1, 1994·Current Opinion in Cell Biology·A Murray

❮ Previous
Next ❯

Citations

Dec 4, 2001·Biology of the Cell·J L MallerB J Tunquist
May 4, 2002·Molecular and Cellular Endocrinology·James L MallerBrian J Tunquist
Jul 28, 2001·Development, Growth & Differentiation·S KurodaA Shinagawa
Sep 12, 2000·Molecular Biology of the Cell·N C KappasJ C Sible
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Satish Kumar AdigaOhtsura Niwa
Jun 28, 2003·Development, Growth & Differentiation·Takeshi Itoh, Atsunori Shinagawa
Mar 6, 2015·PloS One·Maomao ZhangMichael A Lampson
Jun 7, 2005·Developmental Biology·Wen Ling ZhangBrigitte Ciapa
Aug 7, 2004·Developmental Cell·Christopher W ConnJames L Maller
Jan 21, 2017·Genes·Chames KermiDomenico Maiorano
Mar 7, 2001·Current Biology : CB·M S SchwabJ L Maller
Jan 25, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Pablo SabatCarlos López-Pinto
Apr 25, 2018·Molecular Biology of the Cell·Abigail R GerholdPaul S Maddox
May 24, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Reiko ToyamaIgor B Dawid
Jun 4, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bryce A Mendelsohn, Jonathan D Gitlin
Aug 13, 2017·Scientific Reports·Predrag Jevtić, Daniel L Levy
Oct 24, 2000·The Journal of Biological Chemistry·Z XiaP B Carpenter
Aug 30, 2013·The Journal of Biological Chemistry·Christopher M Murphy, W Matthew Michael
Feb 20, 2003·Mechanisms of Development·Ayesha D Carter, Jill C Sible
Mar 6, 2007·Gene·Annalisa MontesantiCarmel Hensey
Jan 1, 2008·Molecular and Cellular Endocrinology·Jérôme Artus, Michel Cohen-Tannoudji
Mar 26, 2003·Genes & Development·Brian J Tunquist, James L Maller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.