Microtubule-dependent distribution of mRNA in adult cardiocytes

American Journal of Physiology. Heart and Circulatory Physiology
Dimitri ScholzGeorge Cooper

Abstract

Synthesis of myofibrillar proteins in the diffusion-restricted adult cardiocyte requires microtubule-based active transport of mRNAs as part of messenger ribonucleoprotein particles (mRNPs) to translation sites adjacent to nascent myofibrils. This is especially important for compensatory hypertrophy in response to hemodynamic overloading. The hypothesis tested here is that excessive microtubule decoration by microtubule-associated protein 4 (MAP4) after cardiac pressure overloading could disrupt mRNP transport and thus hypertrophic growth. MAP4-overexpressing and pressure-overload hypertrophied adult feline cardiocytes were infected with an adenovirus encoding zipcode-binding protein 1-enhanced yellow fluorescent protein fusion protein, which is incorporated into mRNPs, to allow imaging of these particles. Speed and distance of particle movement were measured via time-lapse microscopy. Microtubule depolymerization was used to study microtubule-based transport and distribution of mRNPs. Protein synthesis was assessed as radioautographic incorporation of [3H]phenylalanine. After microtubule depolymerization, mRNPs persist only perinuclearly and apparent mRNP production and protein synthesis decrease. Reestablishing microtubules r...Continue Reading

References

Jan 1, 1987·Annual Review of Physiology·G Cooper
Nov 1, 1974·Journal of Applied Physiology·G Cooper, R M Satava
Jul 1, 1968·Circulation·J Ross, E Braunwald
Jan 1, 1967·Journal of Ultrastructure Research·E Page
Jun 1, 1982·Circulation Research·G Cooper, R J Tomanek
Jul 1, 1981·Circulation·D HuberH P Krayenbuehl
Jun 11, 1995·The American Journal of Physiology·S KatoP J McDermott
Jan 1, 1994·Annual Review of Cell Biology·P Walter, A E Johnson
Aug 11, 1993·The American Journal of Physiology·C T IvesterP J McDermott
Dec 31, 1997·The Journal of Cell Biology·H SatoG Cooper
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G J BassellR H Singer
Mar 27, 1999·The Journal of Biological Chemistry·T NarishigeG Cooper
May 7, 1999·European Journal of Biochemistry·M P Sheetz
Dec 22, 1999·The American Journal of Physiology·Y NagatomoP J McDermott
Mar 22, 2000·Circulation Research·A M GómezG Vassort
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
Mar 27, 2001·Journal of the American College of Cardiology·M R ZileG Cooper
Oct 5, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wenbin MaLaura Schmidt
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Guangmao ChengGeorge Cooper
Nov 21, 2002·Trends in Cell Biology·Kim L Farina, Robert H Singer
Dec 17, 2002·Journal of Applied Physiology·Beatriz S ScopacasaKleber G Franchini
Feb 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Massimo MallardoMichael A Kiebler
May 17, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Yuji IshibashiGeorge Cooper
Jul 12, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Masaru TakahashiGeorge Cooper
Nov 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Guangmao ChengGeorge Cooper
Apr 27, 2005·Nature Reviews. Molecular Cell Biology·Daniel St Johnston
Jun 28, 2005·Biophysical Journal·Anh-Tuan DinhSamir Mitragotri
Oct 18, 2005·Heart Failure Reviews·G Cooper
Dec 15, 2005·Molecular & Cellular Proteomics : MCP·George ElviraWayne S Sossin
May 9, 2006·American Journal of Physiology. Heart and Circulatory Physiology·George Cooper
Jul 11, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nobutaka Hirokawa

❮ Previous
Next ❯

Citations

Jun 16, 2009·American Journal of Physiology. Heart and Circulatory Physiology·John T FassettRobert J Bache
Jun 23, 2009·American Journal of Physiology. Heart and Circulatory Physiology·George Cooper
Nov 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Guangmao ChengGeorge Cooper
Jan 15, 2013·American Journal of Physiology. Heart and Circulatory Physiology·John T FassettRobert J Bache
Jul 16, 2011·International Journal of Cell Biology·Kristen M BartoliWilliam S Saunders
Dec 24, 2010·The Journal of Experimental Biology·Stephen T KinseyRichard M Dillaman
Dec 29, 2009·Biochimica Et Biophysica Acta·Elena S NadezhdinaPavel A Ivanov
Dec 10, 2017·Journal of the American Heart Association·Mathilde R RivaudCarol Ann Remme
Mar 25, 2010·Biochemistry. Biokhimii︠a︡·A Yu Lomakin, E S Nadezhdina
Dec 24, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Ana Gabriela Jimenez, Stephen T Kinsey
Aug 11, 2019·Experimental Biology and Medicine·Matthew A CaporizzoBenjamin L Prosser
Jan 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Stefan HeinJutta Schaper
Mar 13, 2021·Nature Communications·Emily A ScarboroughBenjamin L Prosser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.