Microtubule nucleating gamma-TuSC assembles structures with 13-fold microtubule-like symmetry.

Nature
Justin M KollmanDavid A Agard

Abstract

Microtubules are nucleated in vivo by gamma-tubulin complexes. The 300-kDa gamma-tubulin small complex (gamma-TuSC), consisting of two molecules of gamma-tubulin and one copy each of the accessory proteins Spc97 and Spc98, is the conserved, essential core of the microtubule nucleating machinery. In metazoa multiple gamma-TuSCs assemble with other proteins into gamma-tubulin ring complexes (gamma-TuRCs). The structure of gamma-TuRC indicated that it functions as a microtubule template. Because each gamma-TuSC contains two molecules of gamma-tubulin, it was assumed that the gamma-TuRC-specific proteins are required to organize gamma-TuSCs to match 13-fold microtubule symmetry. Here we show that Saccharomyces cerevisiae gamma-TuSC forms rings even in the absence of other gamma-TuRC components. The yeast adaptor protein Spc110 stabilizes the rings into extended filaments and is required for oligomer formation under physiological buffer conditions. The 8-A cryo-electron microscopic reconstruction of the filament reveals 13 gamma-tubulins per turn, matching microtubule symmetry, with plus ends exposed for interaction with microtubules, implying that one turn of the filament constitutes a microtubule template. The domain structures of...Continue Reading

References

Jan 1, 1991·Biology of the Cell·D Chrétien, R H Wade
Apr 1, 1985·The Journal of Cell Biology·L EvansM Kirschner
Feb 16, 1999·Cell·E NogalesK H Downing
Jun 15, 2000·Nature Cell Biology·T J Keating, G G Borisy
Jun 15, 2000·Nature Cell Biology·H P Erickson
Jan 3, 2001·The Journal of Cell Biology·R N GunawardaneY Zheng
Apr 16, 2002·Molecular Biology of the Cell·Dani B N VinhTrisha N Davis
Jun 5, 2003·Journal of Structural Biology·Joseph A Mindell, Nikolaus Grigorieff
Apr 23, 2004·Biological Procedures Online·Melanie OhiThomas Walz
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Feb 16, 2006·The Journal of Cell Biology·Christel VérolletBrigitte Raynaud-Messina
Apr 7, 2007·Science·Gohta GoshimaNico Stuurman
Jun 26, 2007·Journal of Molecular Biology·Carsten SachseNikolaus Grigorieff
Sep 29, 2007·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Joel QuispeClinton S Potter
Apr 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Luke M RiceDavid A Agard

❮ Previous
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Citations

Oct 29, 2013·Nature Cell Biology·Linda GombosElmar Schiebel
Oct 14, 2011·Nature Reviews. Molecular Cell Biology·Justin M KollmanDavid A Agard
Jul 5, 2011·Nature Structural & Molecular Biology·Valérie GuilletLionel Mourey
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sabine PetryRonald D Vale
Sep 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Po Hien EarStephen W Michnick
Apr 7, 2011·The Journal of Biological Chemistry·Antonio J Martin-GalianoJose M Andreu
Jun 28, 2011·Science·Jamie M KeckMark Winey
Jan 7, 2011·BMC Cell Biology·Sharyn A Endow, Mark A Hallen
Dec 18, 2013·BMC Biology·Julie A Theriot
Jul 18, 2012·Cell Division·Karin Habermann, Bodo Mh Lange
Mar 30, 2011·Journal of Cell Science·Andreas Anders, Kenneth E Sawin
May 17, 2011·PloS One·Tien-chen LinElmar Schiebel
Jan 19, 2012·PloS One·Eileen O'TooleThomas Müller-Reichert
Apr 12, 2012·Genetics·Mark Winey, Kerry Bloom
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Melissa C Hendershott, Ronald D Vale
May 9, 2014·Molecular Biology of the Cell·Pengfei LiuRobert Z Qi
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John V Kilmartin
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark Winey, Eileen O'Toole
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Rosa M Rios
Oct 27, 2010·The Journal of Cell Biology·Franck J FourniolCarolyn A Moores
Dec 8, 2010·The Journal of Cell Biology·Yuk-Kwan ChoiRobert Z Qi
Apr 5, 2012·The Journal of Cell Biology·Sarah ErlemannElmar Schiebel
Sep 1, 2015·Molecular Biology of the Cell·Berl R OakleyYixian Zheng
Jul 21, 2015·Journal of the American Chemical Society·Piotr NowakJianwei Li
Sep 1, 2015·Nature Cell Biology·Sabine Petry, Ronald D Vale
Jun 23, 2015·Nature Cell Biology·Michal WieczorekGary J Brouhard
May 15, 2015·Molecular Biology of the Cell·Yutian PengGeorjana Barnes
Jan 18, 2013·Nucleus·Katja GraumannGeraint Parry
Apr 21, 2016·Cell Cycle·Caroline DelandreAdrian W Moore
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A Montabana, David A Agard
Jan 20, 2015·Nature Structural & Molecular Biology·Justin M KollmanDavid A Agard
May 6, 2016·Annual Review of Biochemistry·Sabine Petry
Jun 22, 2016·The Journal of Cell Biology·Paul T Conduit
May 27, 2016·Molecular Biology of the Cell·Andrew S LyonDavid A Agard
Mar 8, 2017·The Journal of Cell Biology·Raymundo Alfaro-AcoSabine Petry
Mar 3, 2017·Cold Spring Harbor Protocols·Eileen T O'TooleMark Winey
Apr 8, 2014·Current Biology : CB·Eric M LynchKenneth E Sawin
Dec 28, 2016·Cytoskeleton·Juliette Morlon-GuyotWassim Daher
Jan 12, 2017·Protoplasma·Vadym SulimenkoPavel Dráber

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