Microtubule nucleation promoters Mto1 and Mto2 regulate cytokinesis in fission yeast.

Molecular Biology of the Cell
Samantha ER Dundon, Thomas D Pollard

Abstract

Microtubules of the mitotic spindle direct cytokinesis in metazoans but this has not been documented in fungi. We report evidence that microtubule nucleators at the spindle pole body help coordinate cytokinetic furrow formation in fission yeast. The temperature-sensitive cps1-191 strain (Liu et al., 1999) with a D277N substitution in β-glucan synthase 1 (Cps1/Bgs1) was reported to arrest with an unconstricted contractile ring. We discovered that contractile rings in cps1-191 cells constrict slowly and that an mto2S338N mutation is required with the bgs1D277Nmutation to reproduce the cps1-191 phenotype. Complexes of Mto2 and Mto1 with γ-tubulin regulate microtubule assembly. Deletion of Mto1 along with the bgs1D277N mutation also gives the cps1-191 phenotype, which is not observed in mto2S338N or mto1Δ cells expressing bgs1+. Both mto2S338N and mto1Δ cells nucleate fewer astral microtubules than normal and have higher levels of Rho1-GTP at the division site than wild-type cells. We report multiple conditions that sensitize mto1Δ and mto2S338N cells to furrow ingression phenotypes.

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jul 20, 2000·Micron : the International Research and Review Journal for Microscopy·H TatebeM Yanagida
Feb 23, 2002·Nature·V WoodL Cerrutti
Dec 2, 2004·Nature Cell Biology·Sabina ZimmermanFred Chang
Jan 22, 2005·Molecular Biology of the Cell·Itaru SamejimaKenneth E Sawin
Mar 18, 2005·Molecular Biology of the Cell·Sabina Zimmerman, Fred Chang
Oct 15, 2005·Science·Jian-Qiu Wu, Thomas D Pollard
Oct 31, 2006·Yeast·Kenneth E Sawin, P T Tran
Jun 22, 2007·Molecular Microbiology·Juan Carlos G CortésJuan Carlos Ribas
Dec 15, 2007·Science·Dimitrios VavylonisThomas D Pollard
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Jan 14, 2009·The Journal of Cell Biology·Rachel H Roberts-GalbraithKathleen L Gould
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Apr 20, 2010·Nature Cell Biology·Etienne BoulterKeith Burridge
Oct 26, 2010·Current Biology : CB·Itaru SamejimaKenneth E Sawin
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 15, 2012·The Journal of Cell Biology·Juan Carlos G CortésJuan Carlos Ribas
Dec 18, 2013·Genetics·María Alcaide-GavilánJuan Jimenez
May 7, 2014·The Journal of Cell Biology·Ning WangJian-Qiu Wu
Dec 5, 2014·Molecular Biology of the Cell·Reshma DavidsonJian-Qiu Wu
Jan 22, 2015·Journal of Biological Methods·Arthur D EdelsteinNico Stuurman

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Citations

Aug 7, 2020·Molecular Biology of the Cell·Ting Gang ChewMohan K Balasubramanian
Jul 3, 2021·Cells·Jero Vicente-SolerMarisa Madrid

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Methods Mentioned

BETA
biosensor
biosensors
GTPases
PCR
Exponential Fitting

Software Mentioned

SAMtools
MagicBLAST
Micromanager
R
Excel
IGV
ggbeeswarm
FastQC
Therminator
tidyverse

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