Microtubule organization and distribution of gamma-tubulin in male meiosis of lepidoptera

Molecular Reproduction and Development
K W Wolf, H C Joshi

Abstract

Meiotic spindles in males of higher Lepidotera are unusual in that the bulk of the spindle microtubules (MTs) ends about halfway between the equatorial plate and the centrosomes in metaphase. It appears worthwhile to determine how the MTs are nucleated, while their pole proximal ends are distant from the centrosomes. To this end, spermatocytes of Phragmatobia fuliginosa (Arctiidae), collected in the field, were double-labeled with antibodies to beta- and gamma-tubulin. The former antibody reveals the entire microtubular cytoskeleton, and the latter is directed against a newly-discovered tublin isoform that is prevalent in microtubule-organizing centers (MTOCs). The immunocytochemical work was supplemented by a fine structural analysis of MTOCs and spindles. Gamma-tubulin was clearly detected at the spindle poles, and prominent microtubular asters originated from these sites. Additionally, MT arrays at both sides of the equatorial plate in metaphase spermatocytes contained gamma-tubulin. The staining persisted in late anaphase, when kinetochore MTs are depolymerized. This indicates that at least nonkinetochore MTs contain gamma-tubulin. The analysis of ultrathin sections through spindles revealed large amounts of pericentriolar ...Continue Reading

References

Jan 1, 1990·Cell Motility and the Cytoskeleton·R J Leslie
May 1, 1988·The Journal of Cell Biology·M A McNiven, K R Porter
May 1, 1994·The Plant Cell·Z B LiuT J Guilfoyle
Feb 1, 1994·Current Opinion in Cell Biology·H C Joshi
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V T Marchesi, N Ngo

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