PMID: 3745275Sep 1, 1986Paper

Microtubule reassembly from nucleating fragments during the regrowth of amputated neurites

The Journal of Cell Biology
P W Baas, S R Heidemann

Abstract

We have proposed that stable microtubule (MT) fragments that resist depolymerization may serve as nucleating elements for the local control of MT dynamics in the axon (Heidemann, S. R., M. A. Hamborg, S. J. Thomas, B. Song, S. Lindley, and D. Chu, 1984, J. Cell Biol., 99:1289-1295). Here we report evidence that supports this proposal in studies on the role of MTs in the regrowth of neurites from the distal segments of amputated chick sensory neurites. Amputated neurites collapse to "beads" of axoplasm that rapidly regrow (Shaw, G., and D. Bray, 1977, Exp. Cell Res., 104:55-62). We examined both unarrested regrowth and regrowth after MT disassembly by either cold (-5 degrees C for 2 h) or nocodazole (0.1 microgram/ml for 15-20 min). In all these cases regrowth occurred at 3.5-4.5 micron/min with no delay times other than the times to reach 37 degrees C or rinse out the nocodazole. Electron micrographs of untreated beads show many MTs of varying lengths, while those of cold- and nocodazole-treated beads show markedly shorter MTs. The robust regrowth of neurites from beads containing only very short MTs argues against unfurling of intact MTs from the bead into the growing neurite. Electron micrographs of cold-treated beads lysed u...Continue Reading

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Citations

Jan 1, 1990·Cell Motility and the Cytoskeleton·C H Keith
Jan 11, 1992·Cell Motility and the Cytoskeleton·R B DyeR C Williams
Apr 1, 1996·Molecular Neurobiology·P W Baas, W Yu
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Feb 19, 2016·Cytoskeleton·Peter W BaasLanfranco Leo
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