PMID: 8601604Apr 1, 1996Paper

Microtubule transport and assembly during axon growth

The Journal of Cell Biology
W YuP W Baas

Abstract

There is controversy concerning the mechanisms by which the axonal microtubule (MT) array is elaborated, with some models focusing on MT assembly and other models focusing on MT transport. We have proposed a composite model in which MT assembly and transport are both important (Joshi, H.C., and P.W. Baas. 1993. J. Cell Biol. 121:1191-1196). In the present study, we have taken a novel approach to evaluate the merits of this proposal. Biotinylated tubulin was microinjected into cultured neurons that had already grown short axons. The axons were then permitted to grow longer, after which the cells were prepared for immunoelectron microscopic analyses. We reasoned that any polymer that assembled or turned over subunits after the introduction of the probe should label for biotin, while any polymer that was already assembled but did not turnover should not label. Therefore, the presence in the newly grown region of the axon of any unlabeled MT polymer is indicative of MT transport. In sampled regions, the majority of the polymer was labeled, indicating that MT assembly events are active during axon growth. Varying amounts of unlabeled polymer were also present in the newly grown regions, indicating that MT transport also occurs. Toge...Continue Reading

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Citations

Apr 1, 1996·Molecular Neurobiology·P W Baas, W Yu
Mar 11, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Oveis KarimaAli Akbar Moosavi Movahedi
Mar 4, 1998·Current Opinion in Cell Biology·H C Joshi
Mar 4, 1998·Current Opinion in Cell Biology·R A Nixon
Jul 13, 2000·The Journal of Cell Biology·S AckerleyC C Miller
Feb 11, 1999·The Journal of Cell Biology·T J Koehnle, A Brown
Aug 8, 2009·Molecular and Cellular Neurosciences·Fabienne E Poulain, André Sobel
Nov 5, 2003·Journal of Neurobiology·Peter W Baas, Daniel W Buster
May 11, 2011·Developmental Neurobiology·Peter W Baas, Shen Lin
Sep 13, 2002·Current Biology : CB·Lei Wang, Anthony Brown
Apr 16, 2004·Current Biology : CB·Yitao MaSergey V Popov
Aug 9, 2005·Biochimica Et Biophysica Acta·Stéphanie MillecampsJean-Pierre Julien
Jun 16, 2004·Developmental Biology·Juan FernándezAndrea Ubilla
May 4, 2016·The Journal of Cell Biology·Anand N RaoPeter W Baas
Feb 19, 2016·Cytoskeleton·Peter W BaasLanfranco Leo
Jan 29, 2000·Microscopy Research and Technique·P W Baas
Aug 25, 2015·Molecular & Cellular Proteomics : MCP·Atsushi Saito, Valeria Cavalli
Jul 23, 2002·Nature Cell Biology·Yuko FukataKozo Kaibuchi
Mar 20, 2001·Nature Reviews. Molecular Cell Biology·A Brown
May 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tulika SarmaMark M Rasenick
Apr 5, 2021·Neuroscience Letters·Shrobona GuhaPeter W Baas
Aug 22, 2007·Trends in Cell Biology·P W Baas, A Brown
Aug 22, 2007·Trends in Cell Biology·N HirokawaS Takeda
Nov 21, 2017·ACS Chemical Neuroscience·Prasenjit MondalSurajit Ghosh

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