Microtubules and vimentin associated filaments (VIFs) in cultured human gingival fibroblasts (HGFs) after exposure to acrolein and acetaldehyde

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
P PoggiR Boratto

Abstract

Tobacco smoke, particularly its non-volatile fraction e. g. nicotine, is considered to be a major risk factor for the development and progression of periodontal disease. The purpose of this study has been to determine the effects of acrolein and acetaldehyde of the volatile fraction of tobacco smoking, on human gingival fibroblasts (HGFs) cultured in vitro with particular attention to cytoskeletal structures. A human gingival fibroblast strain derived from healthy gingiva was utilized in this study. The cells were exposed to acrolein and acetaldehyde at various concentrations. Control and treated cells were compared as regards their adhesion on cell culture dishes. Their cytoskeletal structures [tubulin and vimentin intermediate filaments (VIFs)] were examined by fluorescence microscopy. The results revealed that both substances produced similar effects resulting in a dose dependent decrease in cell adhesion and alterations of HGF cytoskeleton consisting of rearrangement and/or disruption of microtubules and vimentin associated filaments. Changes in cell shape and decrease in cell size were also seen. On the basis of this in vitro study, it appears that tobacco, through its volatile components, may directly affect the main func...Continue Reading

References

Aug 1, 1996·The Journal of Cell Biology·R D GoldmanP M Steinert
Nov 1, 1996·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·F R CasseeV J Feron
Jan 1, 1996·Archives of Toxicology·F R CasseeV J Feron
Feb 1, 1997·Current Opinion in Cell Biology·Y H ChouR D Goldman
Oct 18, 2000·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·P PoggiR Boratto

❮ Previous
Next ❯

Citations

Nov 9, 2010·Journal of Periodontology·Adrien NaveauBruno Gogly
May 21, 2003·Periodontology 2000·Francisco Rivera-Hidalgo
Jun 6, 2009·Life Sciences·Poonam Sarkar, Barbara E Hayes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.