Microtubules are reorganised and fragmented for uterine receptivity

Cell and Tissue Research
Sadaf N KalamChristopher R Murphy

Abstract

For the development of uterine receptivity, many morphological and molecular changes occur in the apical surface of luminal uterine epithelial cells (UECs) including an increase in vesicular activity. Vesicular movements for exocytosis and endocytosis are dependent on microtubules; however, changes in microtubules in UECs during early pregnancy have received little attention. β-tubulin, one of the main component of microtubules, is distributed throughout the cytoplasm of UECs on day 1 (non-receptive) of pregnancy in the rat. On day 5.5, β-tubulin is concentrated above the nuclei and by day 6 (receptive), β-tubulin is concentrated in a band-like fashion above the nucleus. Western blot analysis of isolated UECs found two bands (50 and 34 kDa) for β-tubulin in UECs during early pregnancy. The intensity of the 34 kDa band was significantly higher on day 6 compared to day 1. The increase in the 34 kDa band may be due to higher proteolytic activity associated with microtubule polymerisation during the receptive state. Transmission electron microscopy showed fragmented microtubules at the time of receptivity in UECs. This is the first study to show that microtubules are reorganised during uterine receptivity. This re-organisation like...Continue Reading

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Citations

May 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Rayees RahmanAvner Schlessinger
Jun 17, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yue ZhangYanqing Geng

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Methods Mentioned

BETA
transmission electron microscopy
protein assay
electrophoresis

Software Mentioned

ZEN
Rad Image Lab
Volume Analysis Tool
GraphPad
GraphPad Prism

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