PMID: 2497465May 1, 1989Paper

Microvascular sites and characteristics of sickle cell adhesion to vascular endothelium in shear flow conditions: pathophysiological implications

Proceedings of the National Academy of Sciences of the United States of America
D K KaulR L Nagel

Abstract

To understand the role of sickle cell adherence to the vascular endothelium in the pathophysiology of sickle cell anemia (SS) vasoocclusion, we have carried out a microcirculatory study utilizing the ex vivo mesocecum vasculature of the rat. A single bolus of washed oxy-normal (AA) erythrocytes or oxy-SS cells (unseparated or density-defined SS cell classes) was infused. Hemodynamic monitoring and intravital microscopic observations of the microvascular flow revealed higher peripheral resistance for SS erythrocytes and adherence of these cells exclusively to the venular endothelium but rare or no adherence of AA cells. The extent of adhesion was inversely correlated with venular diameters (r = -0.812; P less than 0.00001). The adhesion of SS erythrocytes is density-class dependent: reticulocytes and young discocytes (SS1) greater than discocytes (SS2) greater than irreversible sickle cells and unsicklable dense discocytes (SS4). Selective secondary trapping of SS4 (dense cells) is found in postcapillary venules where deformable SS cells are preferentially adhered. We conclude that in the oxygenated condition, vasoocclusion can be induced by two events: (i) random precapillary obstruction by a small number of SS4 cells; (ii) inc...Continue Reading

References

Jul 1, 1987·The Journal of Clinical Investigation·H H LipowskyD M Katz
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C Raventos-SuarezR L Nagel
Jun 21, 1971·Annals of the New York Academy of Sciences·T MatuhasiH Nariuchi
May 1, 1980·The New England Journal of Medicine·R P HebbelM H Steinberg

❮ Previous
Next ❯

Citations

Aug 21, 2010·Cell Biochemistry and Biophysics·Avraham SchlagerDan Arbell
Aug 16, 2000·La Revue de médecine interne·B ChaudetM R Boisseau
Jun 24, 2003·Blood Reviews·Ronald L NagelMartin H Steinberg
Sep 13, 2002·European Journal of Haematology·Pierre Louis TharauxMichel Vayssairat
Feb 13, 2001·British Journal of Haematology·A O EmeribeC Raykundalia
May 25, 2002·Kidney International·Donald E Wesson
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Huan Lei, George E Karniadakis
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M E FabryD K Kaul
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M E FabryF Costantini
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M E FabryR L Nagel
May 7, 2002·Journal of Pediatric Hematology/oncology·Kay L SavingMatthew J Gorman
Feb 15, 2002·Current Opinion in Hematology·Paul S Frenette
Dec 2, 2005·Current Opinion in Hematology·Iheanyi Okpala
May 30, 2002·The British Journal of Ophthalmology·M Kunz MathewsG A Lutty
May 12, 2010·Annual Review of Biomedical Engineering·Gilda A BarabinoDhananjay K Kaul
Jul 1, 1997·American Journal of Respiratory and Critical Care Medicine·S I HammermanH W Farber
Mar 17, 1999·The Journal of Clinical Investigation·S H EmburyA T Cheung
Jul 25, 2006·Blood·Robert S FrancoDonald L Rucknagel
Sep 1, 1991·Journal of Neurosurgery·N M OyesikuA R Colohan
Dec 27, 2011·The Indian Journal of Medical Research·Mohsen A F El-HazmiArjumand S Warsy
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aslihan TurhanPaul S Frenette
Jun 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg GalkinPeter G Vekilov
Jan 15, 1993·Experientia·D K Kaul, R L Nagel
Jan 15, 1993·Experientia·M E Fabry
Feb 27, 2001·Current Opinion in Hematology·R L Nagel
Jan 1, 1990·Hemoglobin·D R Powars
Sep 23, 2014·Expert Opinion on Investigational Drugs·Iheanyi Okpala
Jul 25, 2006·Expert Opinion on Investigational Drugs·Iheanyi Okpala
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·E DuSubra Suresh
Aug 31, 2014·Blood Cells, Molecules & Diseases·P R S CruzM B Melo
Mar 5, 2014·Hematology/oncology Clinics of North America·Abdullah Kutlar, Stephen H Embury
Jul 1, 1996·Kidney International·N BankR L Nagel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.