Microvesicles from Human Adipose Tissue-Derived Mesenchymal Stem Cells as a New Protective Strategy in Osteoarthritic Chondrocytes

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Miguel Tofiño-VianMaria José Alcaraz

Abstract

Chronic inflammation contributes to cartilage degeneration during the progression of osteoarthritis (OA). Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. AD-MSC were isolated by collagenase treatment from adipose tissue from healthy individuals subjected to abdominal lipectomy surgery. Microvesicles and exosomes were obtained from conditioned medium by filtration and differential centrifugation. Chondrocytes from OA patients were used in primary culture and stimulated with 10 ng/ml interleukin(IL)-1β in the presence or absence of AD-MSC microvesicles, exosomes or conditioned medium. Protein expression was investigated by ELISA and immunofluorescence, transcription factor-DNA binding by ELISA, gene expression by real-time PCR, prostaglandin E2 (PGE2) by radioimmunoassay, and matrix metalloproteinase (MMP) activity and nitric oxide (NO) production by fluorometry. In OA chondrocytes stimulated with IL-1β, microvesicles an...Continue Reading

Citations

Nov 19, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Xiaoming LiuThorsten Kirsch
Nov 30, 2019·Current Pharmaceutical Design·Su-Kang ShanLing-Qing Yuan
Nov 15, 2019·Current Pharmaceutical Design·Chao TuZhihong Li
Mar 25, 2020·Cell Transplantation·Bocheng ZhangWeiguo Zhang
Jun 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fernanda O BortolazzoAndrea A de Aro
Sep 29, 2019·Journal of Cellular and Molecular Medicine·YingMei LvQiang Zhang
Jul 1, 2020·Bone Research·Zhenhong NiLin Chen
Sep 17, 2020·Stem Cell Reviews and Reports·Xiaoli QianLisha Li
Nov 7, 2019·International Journal of Molecular Sciences·Magdalena Krajewska-WłodarczykJoanna Wojtkiewicz
Apr 15, 2020·Journal of Extracellular Vesicles·Chang Hee WooYong Woo Cho
Nov 7, 2019·Journal of Clinical Medicine·Daniele D'ArrigoGiuseppe Filardo
Jul 30, 2020·Journal of Clinical Medicine·Houssam AhegetKarim Benabdellah
Aug 15, 2020·Frontiers in Cell and Developmental Biology·Shuyu LiuLifang Hu
May 5, 2020·Frontiers in Bioengineering and Biotechnology·Nathan PiazzaKarin Wuertz-Kozak
Nov 23, 2019·Stem Cell Research & Therapy·Elaheh MianehsazMichael R Hamblin
Sep 11, 2020·Molecular Pain·Simona D'AgnelliLars Arendt-Nielsen
Oct 20, 2020·Frontiers in Cell and Developmental Biology·Mehdi NajarHassan Fahmi
Feb 2, 2020·Acta Biomaterialia·Quan-Fa ZhouXiang-Jin Lin
Dec 10, 2020·International Journal of Molecular Sciences·Luc RochetteCatherine Vergely
Feb 9, 2021·Frontiers in Bioengineering and Biotechnology·Marietta HerrmannSusanne Grässel
Feb 13, 2021·Journal of Personalized Medicine·Loubna MaziniGabriel Malka
Dec 29, 2020·Frontiers in Cell and Developmental Biology·Andrea De LunaStefan Nehrer
Feb 13, 2021·International Journal of Molecular Sciences·María Carmen CarcellerMaría José Alcaraz
Mar 16, 2021·Frontiers in Pharmacology·Wumei WeiXiaohong Tian
Nov 12, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shuyi Xiao, Liang Chen
Mar 31, 2021·Free Radical Biology & Medicine·Cristina Mas-Bargues, Consuelo Borrás

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