Microwave-assisted antigen retrieval and incubation with cox-2 antibody of archival paraffin-embedded human oligodendroglioma and astrocytomas

Journal of Neuroscience Methods
Sehime G TemelLothar Jennes

Abstract

Immunohistochemistry is an important tool that is often used for the diagnosis of pathologies; however, the length of time required to process the tissue is relatively long. Furthermore, the quality and sensitivity of immunohistochemical staining is affected by formalin fixation which results in variable loss of antigenicity, known as masking effect. Here we assess the effect of microwave irradiation on the incubation time required to obtain high quality immunohistochemical staining for cox-2 using archival formalin-fixed, paraffin-embedded human oligodendrogliomas and astrocytomas. The results show that intermittent microwave irradiation during the incubation with the primary antibody reduced the time requirement to 5 min while the staining quality was indistinguishable from 1 or 24 h long incubations. Thus, the use of this procedure results in a significant saving of time which is important for a timely diagnosis of pathological conditions that await treatment.

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Citations

Jul 27, 2007·Journal of Molecular Histology·Delwin J Long, Colleen Buggs
Jul 17, 2013·Journal of Neuroscience Methods·Katrina OwensTibor Kristian
Mar 12, 2013·Journal of Neuroscience Methods·Amanda Bond, John C Kinnamon
Aug 6, 2010·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Bo ZhangElmar Stickeler
Apr 18, 2009·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K KatohS Ookawara

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