PMID: 7536863Dec 1, 1994Paper

Microwave fixation and localization of calcium in synaptic vesicles

Journal of Neuroscience Methods
V MizuhiraM Notoya

Abstract

The distribution of Ca2+ ions is demonstrated in the synaptic terminals by means of a 2-step chemical precipitation of Ca2+ ions in nervous tissue. K-oxalate/K-antimonate chemical replacement with simultaneous computerized microwave irradiation is used. This precipitate in cell structures was investigated by computerized electron probe X-ray micro-analysis. The calculated values (from the theoretical, standards and sections), elemental binding ratios and elemental molecular weight ratios were compared. Each calculated value coincided with the theoretical value. This method can reliably detect Ca2+ ions at the micromolar level. Ca2+ ions were distributed in the synaptic vesicles and surrounding membranes. Further progress is expected in freeze-substitution and in the application and propagation of the EELS-Imaging system in calcium determinations.

References

Aug 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J A Simson, S S Spicer
Oct 1, 1992·Journal of Neurocytology·T TachibanaY Yoshida
Jun 25, 1986·Brain Research·E Fifková, K Cullen-Dockstader
May 1, 1969·The Journal of Cell Biology·M J Legato, G A Langer
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V Mizuhira, M Ueno
May 1, 1984·The Journal of Cell Biology·F UngarE Holtzman
Mar 1, 1984·The Histochemical Journal·M BorgersH E Ter Keurs
Nov 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M Wick, P K Hepler
Sep 1, 1981·The Histochemical Journal·M Borgers

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Citations

Jan 1, 1995·Journal of Physiology, Paris·Y Dunant, M Israël
Jan 4, 2001·Journal of Dermatological Science·T NishiyamaR E Burgeson
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M TsunenagaT Nishiyama
Dec 8, 1998·Micron : the International Research and Review Journal for Microscopy·A S Leong, R T Sormunen
Oct 2, 2009·Journal of Clinical Biochemistry and Nutrition·Naoko KobayashiShiro Urano
Jan 26, 2013·Journal of Magnetic Resonance Imaging : JMRI·Yutong LiuMichael D Boska
Jul 9, 1998·Methods : a Companion to Methods in Enzymology·E Marani

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