PMID: 6105733Mar 1, 1980Paper

Microwave fixation of cells in tissue culture

Stain Technology
M K Patterson, R Bulard

Abstract

Use of microwave irradiation has been evaluated for the in situ fixation of cells grown in tissue culture prior to fluorescent antibody staining. The results show total retention of cell protein in the matrix with microwave fixation, whereas 40-50% of the protein is lost during conventional formaldehyde fixation. Fluorescent antibody staining of cells shows that this protein loss reflects cell loss. A further advantage of microwave fixation is that photometric quantitation of fluorescence appears possible by random scanning of fluorescent antibody stained populations.

References

Mar 1, 1977·Stain Technology·J A Petrere, J L Schardein

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Citations

Mar 1, 1993·Scanning·A S Leong
Aug 1, 1990·Journal of Neuroscience Methods·P J ArmatiF Llewellyn
May 1, 1991·Journal of Virological Methods·J Husson van Vliet
Jan 1, 1980·Toxicology·B Ekwall
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J Barsony, S J Marx
Jun 1, 1990·Journal of Microscopy·L P Kok, M E Boon
Apr 1, 1985·The Histochemical Journal·D Hopwood

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