Jul 18, 2016

MICROWAVE MUTAGENESIS OF BREVIBACILLUS PARABREVIS FOR ENHANCED CELLULASE PRODUCTION, AND INVESTIGATION ON THERMOSTABILITY OF THIS CELLULASE

BioRxiv : the Preprint Server for Biology
Pinakin KhambhalaVijay Kothari

Abstract

Microwave mutagenesis of Brevibacillus parabrevis for enhanced cellulase production was attempted. Though microwave treatment could alter the cellulase activity of the test bacterium, none of the mutants obtained were found to be genetically stable, indicating the reversible nature of microwave-induced mutation(s). Thermal stability of the B. parabrevis cellulase was also investigated. This enzyme was found to be capable of retaining its activity even after heat treatment (50-121°C, for 30-60 min). Fluorescence spectrum revealed a red shift in the emission maxima of the heat-treated enzyme preparations, indicating some structural change upon heating, but no major loss of activity was observed. This enzyme was found to be active over a broad temp range, with 90°C as the optimum temp, which is interesting as the producing organism is a mesophile.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Brevibacillus parabrevis
Other and Unspecified Abnormality of Organs and Soft Tissues of Pelvis, Unspecified as to Episode of Care or Not Applicable
Mutant Proteins
Evaluation
Enzyme agent
Enzyme preparations, digestives
Organism
Structure
Mutant
Cellulase Activity

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Biotechnology and Bioengineering Symposium
E T Reese
Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Ashabil AyganOmer Colak
© 2020 Meta ULC. All rights reserved