Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction

Avian Pathology : Journal of the W.V.P.A
G ElhafiR C Jones

Abstract

A method is described for enabling safe transit of denatured virus samples for polymerase chain reaction (PCR) identification without the risk of unwanted viable viruses. Cotton swabs dipped in avian infectious bronchitis virus (IBV) or avian pneumovirus (APV) were allowed to dry. Newcastle disease virus and avian influenza viruses were used as controls. Autoclaving and microwave treatment for as little as 20 sec destroyed the infectivity of all four viruses. However, both IBV and APV could be detected by reverse transcriptase (RT)-PCR after autoclaving and as long as 5 min microwave treatment (Newcastle disease virus and avian influenza viruses were not tested). Double microwave treatment of IBV and APV with an interval of 2 to 7 days between was tested. After the second treatment, RT-PCR products were readily detected in all samples. Swabs from the tracheas and cloacas of chicks infected with IBV shown to contain infectious virus were microwaved. Swabs from both sources were positive by RT-PCR. Microwave treatment appears to be a satisfactory method of inactivating virus while preserving nucleic acid for PCR identification.

References

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Citations

Oct 7, 2009·The Annals of Occupational Hygiene·Dennis J ViscusiRonald E Shaffer
Feb 13, 2014·Scientific Reports·Veena V UnnithanGeoffrey B Smith
Mar 14, 2007·Zoonoses and Public Health·P De BenedictisI Capua
Feb 5, 2009·Journal of Applied Microbiology·S-Y KimJ-K Park
Jun 12, 2008·Letters in Applied Microbiology·S-Y KimJ-K Park
Jun 28, 2011·The Journal of Eukaryotic Microbiology·Mariana FittipaldiFrancesc Codony
Jun 8, 2012·International Journal of Radiation Biology·Rita Singh, Durgeshwer Singh
Apr 2, 2020·Frontiers in Bioengineering and Biotechnology·David Calderón-FrancoDavid G Weissbrodt
Dec 9, 2020·Avian Pathology : Journal of the W.V.P.A·Damer P Blake
Jul 6, 2021·Journal of Food Processing and Preservation·Reza FarahmandfarBakhtiyar Hesami

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Methods Mentioned

BETA
PCRs
PCR

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