Mid-embryo patterning and precision in Drosophila segmentation: Krüppel dual regulation of hunchback

PloS One
David M Holloway, Alexander V Spirov

Abstract

In early development, genes are expressed in spatial patterns which later define cellular identities and tissue locations. The mechanisms of such pattern formation have been studied extensively in early Drosophila (fruit fly) embryos. The gap gene hunchback (hb) is one of the earliest genes to be expressed in anterior-posterior (AP) body segmentation. As a transcriptional regulator for a number of downstream genes, the spatial precision of hb expression can have significant effects in the development of the body plan. To investigate the factors contributing to hb precision, we used fine spatial and temporal resolution data to develop a quantitative model for the regulation of hb expression in the mid-embryo. In particular, modelling hb pattern refinement in mid nuclear cleavage cycle 14 (NC14) reveals some of the regulatory contributions of simultaneously-expressed gap genes. Matching the model to recent data from wild-type (WT) embryos and mutants of the gap gene Krüppel (Kr) indicates that a mid-embryo Hb concentration peak important in thoracic development (at parasegment 4, PS4) is regulated in a dual manner by Kr, with low Kr concentration activating hb and high Kr concentration repressing hb. The processes of gene express...Continue Reading

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Citations

Sep 27, 2008·PLoS Computational Biology·Francisco J P LopesAlexander V Spirov
Jun 3, 2016·CBE Life Sciences Education·Katherine M StefanskiRebecca L Seipelt-Thiemann
Apr 29, 2016·Journal of Bioinformatics and Computational Biology·Alexander V SpirovDavid M Holloway
Jun 3, 2016·Wiley Interdisciplinary Reviews. Developmental Biology·Jun MaWu-Min Deng
Jul 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thomas R Sokolowski, Gašper Tkačik

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