Mid-IR biosensor: detection and fingerprinting of pathogens on gold island functionalized chalcogenide films

Analytical Chemistry
Chenxu YuJoseph Irudayaraj

Abstract

Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the surface of IR-transparent Ge-containing chalcogenide glass films were formed via thiol chemistry on 20-nm-thick gold islands. As a first step, the protocol was validated by monitoring fluorescently tagged targets to validate binding. FT-IR spectroscopy confirmed that the coating of the films with 20-nm gold did not have a significant effect on the propagation and penetration of IR evanescent waves through the film. The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR fingerprinting. Highly selective detection of bacterial targets was achieved at both the species (E. coli vs. S. enteriditis) and strain level (E. coli O157:H7 vs E. coli K12). A mid-infrared approach could thus be used as a biosensor as well as a molecular fingerprinting tool.

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Citations

Jan 1, 2009·Sensors·Marie-Laure AnneBruno Bureau
Apr 23, 2013·International Journal of Food Microbiology·Il-Hoon Cho, Joseph Irudayaraj
Nov 21, 2007·Analytical Biochemistry·Michèle SalmainClaire-Marie Pradier
Jul 31, 2007·Biophysical Journal·Chenxu Yu, Joseph Irudayaraj
Jan 29, 2013·Analytical and Bioanalytical Chemistry·Il-Hoon Cho, Joseph Irudayaraj
Sep 3, 2013·Chemical Society Reviews·Boris Mizaikoff
Nov 24, 2011·Physical Review Letters·G SubramaniaI Brener
Mar 14, 2009·Analytical Chemistry·Sandeep P RavindranathJoseph Irudayaraj
Jun 6, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Bruno RobertCsilla Gergely
Jun 16, 2007·Science·Carl A Batt

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