Midazolam and Dexmedetomidine Affect Neuroglioma and Lung Carcinoma Cell Biology In Vitro and In Vivo

Anesthesiology
Chunyan WangDaqing Ma

Abstract

WHAT THIS ARTICLE TELLS US THAT IS NEW: BACKGROUND:: Several factors within the perioperative period may influence postoperative metastatic spread. Dexmedetomidine and midazolam are widely used general anesthetics during surgery. The authors assessed their effects on human lung carcinoma (A549) and neuroglioma (H4) cell lines in vitro and in vivo. Cell proliferation and migration were measured after dexmedetomidine (0.001 to 10 nM) or midazolam (0.01 to 400 μM) treatment. Expression of cell cycle and apoptosis markers were assessed by immunofluorescence. Mitochondrial membrane potential and reactive oxygen species were measured by JC-1 staining and flow cytometry. Antagonists atipamezole and flumazenil were used to study anesthetic mechanisms of action. Tumor burden after anesthetic treatment was investigated with a mouse xenograft model of lung carcinoma. Dexmedetomidine (1 nM) promoted cell proliferation (2.9-fold in A549 and 2-fold in H4 cells vs. vehicle, P < 0.0001; n = 6), migration (2.2-fold in A549 and 1.9-fold in H4 cells vs. vehicle, P < 0.0001; n = 6), and upregulated antiapoptotic proteins in vitro. In contrast, midazolam (400 μM) suppressed cancer cell migration (2.6-fold in A549 cells, P < 0.0001; n = 4), induced ...Continue Reading

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Citations

May 31, 2019·Current Opinion in Anaesthesiology·Junichi SaitoDaqing Ma
Aug 28, 2019·Journal of Cellular Biochemistry·Hui YangHao Wu
Oct 22, 2020·International Journal of Immunopathology and Pharmacology·Ewa DomareckaStavros Hatzopoulos
Jan 31, 2021·Biochemical and Biophysical Research Communications·Rongrong YanNa Li
Mar 23, 2021·BMC Anesthesiology·Masashi IshikawaDaqing Ma
May 19, 2021·Journal of Anesthesia·Kiichi Hirota
Oct 13, 2021·Pain Management·Maria F RamirezJuan P Cata

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