Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants

PloS One
Monica K BoruckiJonathan E Allen

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding.

References

Mar 27, 2004·The New England Journal of Medicine·Dongping XuFu-Sheng Wang
Oct 13, 2006·The Journal of General Virology·Astrid VabretFrançois Freymuth
May 29, 2007·Genomics, Proteomics & Bioinformatics·Zhang ZhangJun Yu
Feb 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yu ChenDeyin Guo
Feb 1, 2011·Bioinformatics·Matei DavidMichael Brudno
May 28, 2011·BMC Bioinformatics·Manuel HoltgreweKnut Reinert
May 23, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mark W JackwoodAndreas Handel
Oct 19, 2012·The New England Journal of Medicine·Ali M ZakiRon A M Fouchier
Oct 26, 2012·Journal of Pathogens·Gerard Kian-Meng GohVladimir N Uversky
May 23, 2013·Emerging Infectious Diseases·Matthew CottenEleni Nastouli
Aug 13, 2013·The Lancet Infectious Diseases·Chantal B E M ReuskenMarion P G Koopmans
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Trevor ScobeyRalph S Baric
Nov 26, 2013·PLoS Currents·Gerard Kian-Meng GohVladimir Uversky
Dec 20, 2013·Journal of Virology·Collin TimmJohn Yin
Dec 21, 2013·The Lancet Infectious Diseases·Bart L HaagmansMarion P G Koopmans
Jan 15, 2014·Antiviral Research·Chung-ke ChangTai-huang Huang
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xian-Chun TangWayne A Marasco
Jul 31, 2014·The Journal of Biological Chemistry·Mickaël BouvetXavier Morelli
Jul 31, 2014·Emerging Infectious Diseases·Victor M CormanMarcel A Müller
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Aug 27, 2014·Advances in Bioinformatics·Shea N GardnerMonica K Borucki
Nov 25, 2014·Emerging Infectious Diseases·Danielle R AdneyVincent J Munster
Dec 17, 2014·PLoS Pathogens·Leonid GitlinRaul Andino
Feb 7, 2015·Virus Research·Ian M Mackay, Katherine E Arden

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Citations

Dec 3, 2016·Cold Spring Harbor Molecular Case Studies·Donghyun ParkWoong-Yang Park
Aug 4, 2016·Journal of Medical Virology·Xiaoyan LuDean D Erdman
Dec 14, 2019·Journal of Infection and Public Health·Sayed S Sohrab, Esam I Azhar
Jun 8, 2021·Frontiers in Veterinary Science·Herbert F JelinekHabiba S Alsafar

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Datasets Mentioned

BETA
PRJNA302555

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

SHRiMP2
Blast
KaKs
Excel
Blastp
fastq
mcf

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