Midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible, and field-caught populations of Plutella xylostella, and phenomics of the predominant midgut bacterium Enterococcus mundtii

Scientific Reports
Wenhong LiFengliang Li

Abstract

Gut bacteria play a significant role in host insect. This study evaluated detail difference of midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible and field-caught populations of diamondback moth, and studied phenomics of the predominant midgut bacterium Enterococcus mundtii. Cultivable bacteria revealed that E. mundtii and Carnobacterium maltaromaticum dominated the bacterial populations from deltamethrin-resistant and deltamethrin-susceptible larval midguts, whereas E. mundtii was predominant in field-caught population. Illumina sequencing analysis indicated that 97% of the midgut bacteria were from the phyla Firmicutes, Proteobacteria and Cyanobacteria. Both resistant and susceptible populations had more Enterococcus and Carnobacterium. Enterococcus, Carnobacterium, Bacillus, and Pseudomonas were predominant in the field-caught population. A phenomics analysis revealed that E. mundtii was able to metabolize 25.26% of the tested carbon sources, 100% of the nitrogen sources, 100% of the phosphorus sources and 97.14% of the sulfur sources, had a wide range of osmolytes and pH conditions, and showed active deaminase activity but no decarboxylase activity. This is the first report regarding different populations o...Continue Reading

References

May 29, 2003·Insect Biochemistry and Molecular Biology·D M Soderlund, D C Knipple
Dec 4, 2003·Annual Review of Entomology·R J Dillon, V M Dillon
Jan 9, 2004·Applied and Environmental Microbiology·Nichole A BroderickJo Handelsman
Dec 17, 2004·Journal of Bacteriology·Lisa M MaurerJoan L Slonczewski
Jan 1, 1984·Applied and Environmental Microbiology·K L IversonT P Freeman
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Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yoshitomo KikuchiTakema Fukatsu
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Jan 8, 2013·Applied and Environmental Microbiology·Yoshitomo Kikuchi, Isao Yumoto

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Datasets Mentioned

BETA
KT723002

Methods Mentioned

BETA
Illumina sequencing
PCR

Software Mentioned

BLASTN
Vegan
OmniLog
SPSS
UCHIME
R
Biolog Kinetic and Parametric
Biolog OmniLog
UPARSE

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