Midgut de novo transcriptome analysis and gene expression profiling of Spodoptera exigua larvae exposed with sublethal concentrations of Cry1Ca protein.

3 Biotech
Xiang-Liang RenYan Ma

Abstract

Spodoptera exigua (Hübner) is a polyphagous pest on agricultural crops, whose control is based mainly on the application of chemical insecticides. Bacillus thuringiensis (Bt) is one of the most important biological agents that have been successfully applied as a biological control, and Cry1Ca protein is considered to be active against S. exigua. Therefore, to understand the response of S. exigua to Cry1Ca protein, high-throughput sequencing was used to analyse the S. exigua larval midgut after treatment with sublethal concentrations of Cry1Ca protein. Transcriptome data showed that a total of 98,571 unigenes with an N50 value of 1135 bp and a mean length of 653 bp were obtained. Furthermore, 2962 differentially expressed genes (DEGs) were identified after Cry1Ca challenge, including 1508 up-regulated and 1454 down-regulated unigenes. Among these DEGs, detoxification (CYP, CarE, and GST) and Bt resistance (ALP, APN, and ABC transporter)-related genes were differentially expressed in the midgut of S. exigua after Cry1Ca treatment. However, most DEGs of protective enzymes were down-regulated, while most DEGs related with serine protease and REPAT were up-regulated. Furthermore, almost all DEGs related to the immune signaling pathw...Continue Reading

References

Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·N StrizhovA Zilberstein
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·M Mahbubur RahmanOtto Schmidt
Jun 18, 2004·Immunological Reviews·Michael R KanostXiao-Qiang Yu
May 16, 2006·Immunobiology·Pikul JiravanichpaisalKenneth Söderhäll
Sep 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nichole A BroderickJo Handelsman
Jan 11, 2007·Archives of Insect Biochemistry and Physiology·R S HakimG Smagghe
Jun 7, 2007·Insect Biochemistry and Molecular Biology·Archana S GandheJavaregowda Nagaraju
Oct 6, 2007·Insect Biochemistry and Molecular Biology·Salvador HerreroRuud A de Maagd
Dec 18, 2007·Journal of Invertebrate Pathology·Patricia Hernández-MartínezBaltasar Escriche
Jan 31, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xuebin ZhangLee A Bulla
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Oct 16, 2009·Molecular Immunology·Michael ChapelleBernard Duvic
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Angeles Cancino-RodeznoAlejandra Bravo
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Mar 13, 2012·Insect Molecular Biology·G Navarro-CerrilloS Herrero
May 9, 2012·Insect Biochemistry and Molecular Biology·Laura PascualSalvador Herrero
Oct 6, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G Navarro-CerrilloS Herrero
Sep 24, 2013·Journal of Invertebrate Pathology·Youngjin Park, Yonggyun Kim
May 9, 2014·Applied Microbiology and Biotechnology·Hui-Yu YiXiao-Qiang Yu
Sep 30, 2014·Critical Reviews in Biotechnology·André Luiz de Almeida MeloCarlos Ricardo Soccol
Jul 21, 2016·Current Opinion in Insect Science·Salvador HerreroJuan Ferré

❮ Previous
Next ❯

Related Concepts

Related Feeds

ABC Transporters & Multidrug Resistance

ABC Transporters or ATP-Binding Cassette Transporters are responsible for pumping out antibiotics in the cell and can lead to multidrug resistance in bacteria. Discover the latest research on ABC Transporters & Multidrug Resistance here.