Midkine confers Adriamycin resistance in human gastric cancer cells.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Ying-ying XuFeng Jin

Abstract

Midkine (MDK) is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis, which is overexpressed in most of human malignant tumors and may act as an oncoprotein. The aim of the current study was to investigate the mechanism of MDK involved in the Adriamycin (ADR) resistance in human gastric cancer cells in vitro. We found that Adriamycin-resistant SGC7901 (SGC7901/ADR) exhibited 58.6-fold greater resistance to ADR compared with Adriamycin-sensitive SGC7901 cell line. MDK mRNA and protein expression levels were significantly higher in SGC7901/ADR than in SGC7901. To gain a deeper insight into the role of MDK in SGC7901/ADR, we stably transfected Adriamycin-sensitive SGC7901 with viral vector expressing MDK. Our result showed that multidrug resistance type I (MDR1) was found in SGC7901/ADR, not in SGC7901 by RT-PCR regardless of MDK transfection. P-Glycoprotein, which is the MDR1-coded protein, was found in SGC7901/ADR, not in SGC7901 by Western blot regardless of MDK transfection. We investigated whether an activation of the tyrosine kinase pathway would change the drug resistance phenotype with MDK transfection. Western blot results showed the upregulation of phosphorylated prote...Continue Reading

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Citations

Jan 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Bin HanBin Wu
Oct 15, 2013·British Journal of Pharmacology·S Kishida, K Kadomatsu
Dec 23, 2014·Future Oncology·Chunmei GuoMing-Zhong Sun
Dec 6, 2019·Oncogene·Panagiota S FilippouAnastasia Constantinidou
Jan 19, 2018·International Journal of Molecular Medicine·Yongliang LuYing Bao
Jul 14, 2018·Journal of Experimental & Clinical Cancer Research : CR·Juan LuXiang-Ping Li

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