Abstract
We examined possible application of a regulatory region of midkine (MK) gene, which is frequently upregulated in a number of human tumours but not in normal cells, to cancer gene therapy. We examined transcriptional activity of the MK genomic fragments in paired cell lines, immortalized cells and their parental normal fibroblasts, and found that the MK fragments activated a fused reporter or a suicide gene preferentially in the immortalized cells. Recombinant adenoviruses (Ad), in which the MK fragment was inserted upstream to the E1A gene (AdMK), replicated preferentially in the immortalized cells and were cytotoxie to them. Human hepatocellular carcinoma cells were significantly susceptible to AdMK compared with human normal fibroblasts in vitro and the replication of AdMK was less than that of wild-type Ad in the infected fibroblasts. Hepatocellular carcinoma cells infected with AdMK did not form tumours in immunocompromised mice and intratumoural injection of AdMK into the hepatocellular carcinoma developed in mice retarded the subsequent tumour growth. Expression of E1A and necrosis of tumours were detected in AdMK-injected but not control Ad-injected cases. The MK promoter-driven suicide gene therapy and -mediated replica...Continue Reading
References
Mar 30, 1988·Biochemical and Biophysical Research Communications·K KadomatsuT Muramatsu
Jul 1, 1995·Japanese Journal of Cancer Research : Gann·K AridomeT Muramatsu
Jan 6, 1997·International Journal of Cancer. Journal International Du Cancer·K FushimiM Namba
Oct 24, 1997·In Vitro Cellular & Developmental Biology. Animal·K KinoM Namba
Feb 23, 2000·Journal of Virology·D M ShayakhmetovA Lieber
Jul 11, 2000·Nature Biotechnology·R AlemanyD T Curiel
Mar 27, 2001·International Journal of Cancer. Journal International Du Cancer·M MiyauchiM Tagawa
Jul 7, 2001·Human Gene Therapy·D HarrisonJ G Hay
Jan 5, 2002·Cancer Gene Therapy·T MaedaM Tagawa
Oct 25, 2002·Human Gene Therapy·Ruben Hernandez-AlcocebaMichael F Clarke
Jul 5, 2003·Virology·P Seshidhar ReddySheila Connelly
Jul 15, 2003·Gene Therapy·T-G HuangS L C Woo
May 13, 2004·Oncology·Ling YuMasatoshi Tagawa
Citations
May 13, 2006·Cancer Immunology, Immunotherapy : CII·Masatoshi TagawaTakenori Ochiai
Jun 28, 2005·Biochemical and Biophysical Research Communications·Laura EzquerraThomas F Deuel
Jan 1, 1997·Journal of Neuroscience Methods·H J FederoffT Corden
Jun 12, 2001·Biochemical Systematics and Ecology·S D. SarkerM S.J. Simmonds
Dec 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christopher M HineVera Gorbunova
Sep 27, 2014·BMC Cancer·Takeo SuzukiMasatoshi Tagawa
Aug 8, 2015·Cancer Gene Therapy·Y TakeiM Tagawa
Aug 23, 2008·Journal of Experimental & Clinical Cancer Research : CR·Eiji ToyodaShinji Uemoto
May 20, 2006·Neurosurgical Focus·Weijun WangThomas C Chen
Jun 11, 2015·BMC Cancer·Shan YangMasatoshi Tagawa
Jan 1, 2010·Cancers·Hirofumi Jono, Yukio Ando
Mar 20, 2018·Cell Death & Disease·Yu ChenZhiyong Mao
Nov 12, 2017·Virology Journal·Takao MorinagaMasatoshi Tagawa
Jan 18, 2006·Reviews in Medical Virology·Adam M SonabendMaciej S Lesniak
Dec 6, 2019·Oncogene·Panagiota S FilippouAnastasia Constantinidou
Apr 20, 2014·Oncotarget·Yan CaoZhiyong Mao
Sep 19, 2006·World Journal of Gastroenterology : WJG·Tie-Jun LiAi-Long Huang
Sep 7, 2017·BMC Cancer·Suguru YamauchiMasatoshi Tagawa
Feb 25, 2021·IUBMB Life·Arezoo Gowhari ShabgahJamshid Gholizadeh Navashenaq