MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Journal of Extracellular Vesicles
Joshua A WelshJennifer C Jones

Abstract

Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented many challenges and has produced a number of controversial results, in part due to limitations of instrument detection, lack of robust methods and ambiguities in how data should be interpreted. These complications are exacerbated by the field's lack of a robust reporting framework, and many EV-FC manuscripts include incomplete descriptions of methods and results, contain artefacts stemming from an insufficient instrument sensitivity and inappropriate experimental design and lack appropriate calibration and standardization. To address these issues, a working group (WG) of EV-FC researchers from ISEV, ISAC and ISTH, worked together as an EV-FC WG and developed a consensus framework for the minimum information that should be provided regarding EV-FC. This framework incorporates the existing Minimum Information for Studies of EVs (MISEV) guidelines and Minimum Information about a FC experiment (MIFlowCyt) standard in an EV-FC-specific reporting framework (MIFlowCyt-EV) that supports r...Continue Reading

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Citations

May 12, 2020·Advanced Biosystems·Nicklas HamiltonFerdinando Pucci
Jun 2, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Joshua A WelshVera A Tang
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Methods Mentioned

BETA
flow
Flow Cytometry
size-exclusion chromatography

Software Mentioned

Flow
Mie
FCM PASS
MIFlowCyt
TRACK
MISEV
SR
FlowRepository
EV

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