Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation

Nature Cell Biology
Dong JiangLi Yu

Abstract

Migrasomes are recently identified vesicular organelles that form on retraction fibres behind migrating cells. Whether migrasomes are present in vivo and, if so, the function of migrasomes in living organisms is unknown. Here, we show that migrasomes are formed during zebrafish gastrulation and signalling molecules, such as chemokines, are enriched in migrasomes. We further demonstrate that Tspan4 and Tspan7 are required for migrasome formation. Organ morphogenesis is impaired in zebrafish MZtspan4a and MZtspan7 mutants. Mechanistically, migrasomes are enriched on a cavity underneath the embryonic shield where they serve as chemoattractants to ensure the correct positioning of dorsal forerunner cells vegetally next to the embryonic shield, thereby affecting organ morphogenesis. Our study shows that migrasomes are signalling organelles that provide specific biochemical information to coordinate organ morphogenesis.

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Citations

Jul 3, 2020·Frontiers in Cell and Developmental Biology·Yaxing ZhangHongzhi Yang
Aug 18, 2020·Cell Discovery·Puzhong LuYang Chen
Aug 3, 2019·Nature Cell Biology·Stefania Tavano, Carl-Philipp Heisenberg
Nov 24, 2020·The European Physical Journal. Special Topics·Irep Gözen, Paul Dommersnes
Mar 2, 2021·The EMBO Journal·Diégo Cordero Cervantes, Chiara Zurzolo
Mar 24, 2021·Developmental Cell·Tamako NishimuraShiro Suetsugu
Apr 3, 2021·Development·Aasakiran MadamanchiDavid M Umulis
Apr 4, 2021·Developmental Cell·Bong Hwan SungAlissa M Weaver
Aug 12, 2019·Trends in Cell Biology·Frederik J VerweijJacky G Goetz
Jun 3, 2021·Cell Metabolism·Douglas R Green
Aug 7, 2021·American Journal of Medical Genetics. Part a·Georgia PitsavaUNKNOWN University of Washington Center for Mendelian Genomics, NISC Comparative Sequencing Program and the National Birth Defects P
Aug 28, 2021·Nature Methods·Frederik J VerweijGuillaume van Niel
Aug 28, 2021·Biomolecules·Xiaoyuan MaHenry F Vischer
Aug 27, 2021·Development·Aasakiran MadamanchiDavid M Umulis
Sep 18, 2021·Journal of Extracellular Vesicles·Kirsi Rilla
Sep 8, 2021·The FEBS Journal·Shunbang Yu, Li Yu
Nov 25, 2021·Journal of Extracellular Vesicles·Alin RaiDavid W Greening

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Methods Mentioned

BETA
confocal microscopy
transmission electron microscopy
transgenic
PCR

Software Mentioned

Image J
GraphPad Prism5
Imaris
Gene
SEQUEST
Proteome Discoverer
Excel

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