Migration in Confined 3D Environments Is Determined by a Combination of Adhesiveness, Nuclear Volume, Contractility, and Cell Stiffness

Biophysical Journal
Lena A LautschamBen Fabry

Abstract

In cancer metastasis and other physiological processes, cells migrate through the three-dimensional (3D) extracellular matrix of connective tissue and must overcome the steric hindrance posed by pores that are smaller than the cells. It is currently assumed that low cell stiffness promotes cell migration through confined spaces, but other factors such as adhesion and traction forces may be equally important. To study 3D migration under confinement in a stiff (1.77 MPa) environment, we use soft lithography to fabricate polydimethylsiloxane (PDMS) devices consisting of linear channel segments with 20 μm length, 3.7 μm height, and a decreasing width from 11.2 to 1.7 μm. To study 3D migration in a soft (550 Pa) environment, we use self-assembled collagen networks with an average pore size of 3 μm. We then measure the ability of four different cancer cell lines to migrate through these 3D matrices, and correlate the results with cell physical properties including contractility, adhesiveness, cell stiffness, and nuclear volume. Furthermore, we alter cell adhesion by coating the channel walls with different amounts of adhesion proteins, and we increase cell stiffness by overexpression of the nuclear envelope protein lamin A. Although ...Continue Reading

References

Oct 1, 1992·The Journal of Cell Biology·J L Rodríguez FernándezA Ben-Ze'ev
Apr 4, 1998·The Journal of Experimental Medicine·D FengA M Dvorak
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·N WangD E Ingber
Sep 7, 2001·Current Opinion in Cell Biology·B Geiger, A Bershadsky
Feb 8, 2002·American Journal of Physiology. Cell Physiology·James P ButlerJeffrey J Fredberg
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Apr 2, 2003·Cell Biology Education·Bruce Alberts, UNKNOWN National Academies
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melanie RolliBrunhilde Felding-Habermann
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jun 7, 2005·Nature Materials·Predrag BursacJeffrey J Fredberg
Jul 11, 2006·The Journal of Biological Chemistry·Jan LammerdingRichard T Lee
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Muhammad H ZamanPaul Matsudaira
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Carina RaupachBen Fabry
Dec 22, 2007·Biophysical Journal·Claudia Tanja MierkeBen Fabry
Jan 12, 2008·Journal of Cell Science·Martin W GoldbergReimer Stick
May 3, 2008·Nature·Tim LämmermannMichael Sixt
May 23, 2008·Molecular Biology of the Cell·Christopher BeadlePeter Canoll
Jul 26, 2008·Nature Nanotechnology·Sarah E CrossJames K Gimzewski
Nov 7, 2008·Histochemistry and Cell Biology·Stephanie AlexanderPeter Friedl
Nov 22, 2008·Biochemical Society Transactions·Martin W GoldbergReimer Stick
Feb 19, 2009·Cancer Research·Torsten W RemmerbachJochen Guck
Mar 5, 2009·Physical Review Letters·R J HawkinsR Voituriez
Jun 6, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Mathieu-Benoît VoisinSussan Nourshargh
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Démosthène MitrossilisAtef Asnacios
Feb 26, 2010·The Journal of Biological Chemistry·Claudia T MierkeBen Fabry
Nov 27, 2010·Current Opinion in Cell Biology·Peter FriedlJan Lammerding
Jan 13, 2011·Journal of Cell Science·Claudia Tanja MierkeBen Fabry
Nov 1, 2011·Trends in Cell Biology·Katarina Wolf, Peter Friedl
Apr 6, 2012·PloS One·Thorsten M KochBen Fabry
Nov 14, 2012·Journal of Theoretical Biology·Marco Scianna, Luigi Preziosi
Oct 15, 2013·Immunological Reviews·Mélina L HeuzéAna-Maria Lennon-Duménil
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kristin SeltmannThomas M Magin
Jun 26, 2015·Nature Communications·Claus MetznerBen Fabry

❮ Previous
Next ❯

Citations

Feb 20, 2016·Current Opinion in Cell Biology·Alexandra Lynn McGregorJan Lammerding
Dec 8, 2015·Nature Methods·Julian SteinwachsBen Fabry
Apr 9, 2016·Cells·Jing Ling HoonCheng-Gee Koh
Apr 16, 2016·Current Opinion in Cell Biology·Ryan J Petrie, Kenneth M Yamada
Mar 11, 2016·Nucleus·Richik N MukherjeeDaniel L Levy
Sep 10, 2016·Experimental Cell Research·Lingling LiuGuanbin Song
Aug 9, 2016·Annual Review of Cell and Developmental Biology·Ewa K PaluchMichael Sixt
Mar 24, 2017·ACS Nano·Moustafa R K AliMostafa A El-Sayed
Mar 1, 2017·Biology of the Cell·Charlotte AlibertJean-Baptiste Manneville
Feb 23, 2017·Annals of Biomedical Engineering·Yulia Merkher, Daphne Weihs
Oct 11, 2017·Cells·Ilaria PecorariOrfeo Sbaizero
Nov 7, 2017·Advanced Healthcare Materials·Enrico Domenico LemmaFerruccio Pisanello
Oct 21, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Angelyn V NguyenAmy C Rowat
Oct 6, 2017·Cell Biology International·Wolfgang H Goldmann
Sep 28, 2018·Cell Adhesion & Migration·Alexandra SneiderDong-Hwee Kim
Nov 5, 2016·Journal of Biomechanical Engineering·Marianne LintzCynthia A Reinhart-King
Jan 9, 2019·Nature Reviews. Rheumatology·Feini QuRobert L Mauck
Feb 15, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sreya DasRahul Purwar
Sep 15, 2019·Nature Communications·Matthew R ZanotelliCynthia A Reinhart-King
Nov 30, 2019·Nature Reviews. Cancer·Gautier FollainJacky G Goetz
Dec 24, 2019·Advanced Healthcare Materials·Kwang Hoon SongJason A Burdick
Jul 11, 2020·Biotechnology and Bioengineering·Manasa GaddeMarissa N Rylander
Jan 8, 2020·Biomechanics and Modeling in Mechanobiology·Adrian Moure, Hector Gomez
May 8, 2018·Nature Communications·Christoph MarkBen Fabry
Jul 12, 2019·Frontiers in Cell and Developmental Biology·Navjot Kaur GillAmy C Rowat
Aug 23, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Noam Zuela-Sopilniak, Jan Lammerding
Aug 23, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marina KrauseKatarina Wolf
Apr 1, 2020·Frontiers in Cell and Developmental Biology·Naotaka Nakazawa, Mineko Kengaku
Jun 16, 2016·Tissue Engineering. Part B, Reviews·Shantanu PradhanElizabeth A Lipke
Mar 12, 2019·Wiley Interdisciplinary Reviews. Computational Molecular Science·H G Othmer
Mar 8, 2019·Biomechanics and Modeling in Mechanobiology·S Hervas-RaluyM J Gomez-Benito
Jun 7, 2020·Cell Communication and Signaling : CCS·Tim HohmannFaramarz Dehghani
Mar 16, 2018·Frontiers in Cell and Developmental Biology·Josette M NorthcottValerie M Weaver
Nov 9, 2018·Biophysics Reviews·Vassilis PapalazarouLaura M Machesky
Jun 11, 2020·Cancer Cell International·Sangwoo KwonKyung Sook Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Related Papers

Lab on a Chip
Phrabha S RamanKonstantinos Konstantopoulos
Current Opinion in Cell Biology
Sharona Even-Ram, Kenneth M Yamada
European Journal of Cell Biology
Claudia Tanja MierkeJan Brábek
© 2021 Meta ULC. All rights reserved