Migration inhibitory factor-related protein (MRP)8 and MRP14 are differentially expressed in free-electron laser and scalpel incisions

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Nanjun Wu, Jeffrey M Davidson

Abstract

Incisions made in mouse skin by scalpel or the free-electron laser heal at different rates. To identify genes that are differentially expressed in free-electron laser or scalpel wounds, we isolated total RNA from free-electron laser- or scalpel-produced incisions and normal skin at day 7 postwounding. cDNA microarray analysis identified 89 of 15,000 genes in a mouse microarray as having significantly different expression levels. Migration inhibitory factor-related protein (MRP) 14 was almost 30 times more highly expressed in scalpel wounds than in free-electron laser wounds. This result was confirmed by Northern blot analysis, which also showed that scalpel wounds expressed higher levels of MRP8, a related S100 protein that can heterodimerize with MRP14, at days 2, 7, and 14 postwounding. Free-electron laser wounds also showed elevated expression of MRP8 and MRP14 relative to normal skin. In situ hybridization showed that the patterns of MRP14 and MRP8 expression in free-electron laser and scalpel wound tissues were similar. MRP14 and MRP8 were expressed in the dermal wound margin, while a very low level of MRP14 and MRP8 expression was seen in the migrating epidermis. Dual immunofluorescence staining for MRP14 or MRP8 and macr...Continue Reading

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Citations

Mar 10, 2010·Amino Acids·Jesse Goyette, Carolyn L Geczy
Aug 2, 2012·The Journal of Clinical Investigation·James K ChanJagdeep Nanchahal
Apr 30, 2009·Journal of Leukocyte Biology·Jyotika SharmaJudy M Teale
Jun 7, 2011·Journal of Biomedical Optics·Mark A MackanosChristopher H Contag
Mar 5, 2013·Analytical Chemistry·Kelly M HinesJohn A McLean
Feb 20, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Robert L CaldwellLillian B Nanney
Dec 15, 2015·Journal of Clinical Periodontology·Suzanne L DelimaDimitris N Tatakis
Jan 3, 2012·Respiratory Medicine·Atsuko HaraShigeru Kohno
Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Claus MoserPeter Østrup Jensen
Mar 10, 2020·BioMed Research International·Ryosuke TakagiHiromichi Yumoto
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Su Yin LimCarolyn L Geczy
Mar 12, 2021·Frontiers in Immunology·Claus MoserTim Tolker-Nielsen

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