Migration of Bacteriocins Across Gastrointestinal Epithelial and Vascular Endothelial Cells, as Determined Using In Vitro Simulations

Scientific Reports
Leané DreyerAnton D van Staden

Abstract

Little is known about the migration of bacteriocins across human cells. In this study, we report on migration of three bacteriocins nisin, plantaricin 423 and bacST4SA across colonic adenocarcinoma (Caco-2) cells and human umbilical vein endothelial cells (HUVECs). Bacteriocins were fluorescently labelled while still maintaining antimicrobial activity. Migration of fluorescently labelled bacteriocins across monolayers was assessed in vitro using transmigration well inserts. After 3 h, 75% of nisin, 85% of plantaricin 423 and 82% of bacST4SA migrated across the Caco-2 cell monolayer. Over the same time span, 88% nisin, 93% plantaricin 423 and 91% bacST4SA migrated across the HUVEC monolayer. The viability of both cell types remained unchanged when exposed to 50 µM of nisin, plantaricin 423 or bacST4SA. The effect of human plasma on bacteriocin activity was also assessed. Activity loss was dependent on bacteriocin type and concentration, with the class-IIa bacteriocins retaining more activity compared to nisin. This is the first report of bacteriocins migrating across simulated gastrointestinal- and vascular-barriers. This study provides some of the first evidence that bacteriocins are capable of crossing the gut-blood-barrier. H...Continue Reading

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Citations

Jul 14, 2020·Frontiers in Bioengineering and Biotechnology·Lamiaa A Al-MadbolyAmany E Ragab
Sep 26, 2020·Frontiers in Microbiology·Santosh Kumar TiwariMichael L Chikindas
Sep 3, 2020·FEMS Microbiology Reviews·Samira SoltaniIsmail Fliss
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May 9, 2021·Applied and Environmental Microbiology·Anton Du Preez van StadenCarine Smith
May 22, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·James FlynnSarah P Hudson
Jul 25, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Stine Egebro BirkLine Hagner Nielsen

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Methods Mentioned

BETA
confocal microscopy
protein assay

Software Mentioned

ImageJ
GraphPad Prism

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