PMID: 3765366Jun 1, 1986Paper

Migration of fish leucocytes in vitro: the effect of factors which may be involved in mediating inflammation

Veterinary Immunology and Immunopathology
K A NashA W Thomson

Abstract

Plaice (Pleuronectes platessa L.) neutrophils were isolated from the kidney on a discontinuous Percoll gradient and from the peritoneal cavity at the peak of a glycogen-elicited inflammatory response. The migratory ability of neutrophils was assessed using a 48-well microchemotaxis chamber, with an incubation of 1.5 h at 12 degrees C. The two neutrophil populations showed different responses to N-formylmethionyl-leucyl-phenylalanine (FMLP). Whereas kidney neutrophils only showed a significant enhancement of migration at 10(-7) M, inflammatory neutrophils exhibited a bimodal response, with one peak of migratory activity at 10(-9) M and a second at greater than 10(-6) M. Kidney neutrophils showed a consistent response with various concentrations of a 24 h culture supernatant of Vibrio alginolyticus. In every case increased migration was observed with 5-, 10- and 100-fold dilutions, with the latter two conditions producing a significant enhancement (p less than 0.01 and p less than 0.05 respectively). The undiluted and 2-fold diluted supernatant caused a decreased cell migration compared with control values. The supernatant from kidney neutrophils cultured with serum-opsonized, heat-killed V. alginolyticus produced greater migrato...Continue Reading

References

Jan 1, 1985·Developmental and Comparative Immunology·S D Obenauf, S H Smith
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R M ClancyT E Hugli

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Citations

Jan 1, 1990·Developmental and Comparative Immunology·J F SövényiR Kusuda
Oct 1, 1993·Veterinary Immunology and Immunopathology·M L Cross, R A Matthews
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K A NashA W Thomson
Jan 1, 1991·Biotechnology Advances·N C Bols
Mar 22, 2008·Journal of Fish Diseases·A A WiedenmayerC A Shoemaker

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