Jan 1, 1974

Migration of glycoprotein from the Golgi apparatus to the surface of various cell types as shown by radioautography after labelled fucose injection into rats

The Journal of Cell Biology
G BennettA Haddad


A single intravenous injection of L-[(3)H]fucose, a specific glycoprotein precursor, was given to young 35-45 g rats which were sacrificed at times varying between 2 min and 30 h later. Radioautography of over 50 cell types, including renewing and nonrenewing cells, was carried out for light and electron microscope study. At early time intervals (2-10 min after injection), light microscope radioautography showed a reaction over nearly all cells investigated in the form of a discrete clump of silver grains over the Golgi region. This reaction varied in intensity and duration from cell type to cell type. Electron microscope radioautographs of duodenal villus columnar cells and kidney proximal and distal tubule cells at early time intervals revealed that the silver grains were restricted to Golgi saccules. These observations are interpreted to mean that glycoproteins undergoing synthesis incorporate fucose in the saccules of the Golgi apparatus. Since fucose occurs as a terminal residue in the carbohydrate side chains of glycoproteins, the Golgi saccules would be the site of completion of synthesis of these side chains. At later time intervals, light and electron microscope radioautography demonstrated a decrease in the reaction i...Continue Reading

  • References35
  • Citations136


  • References35
  • Citations136


Mentioned in this Paper

Intestinal Villus
Golgi Apparatus
Intravenous Injections
Tissue Specificity
Lymphoid Cells
Renal Tubule Structure

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